Antiproliferative and antioxidant potential of beta-ionone against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice
Antiproliferative and antioxidant potential of beta-ionone against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice
复制标题
DOI：
10.1007/s11010-011-1186-6
复制
发表时间：
2012-04-01
影响因子：
4.3
通讯作者：
Devaki, Thiruvengadam
中科院分区：
文献类型：
--
作者：
Asokkumar, Selvamani;Naveenkumar, Chandrashekar;Devaki, Thiruvengadam
如今，在像印度这样的发展中国家，肺癌的发病率正在迅速上升，因此它已经成为与恶性肿瘤相关的最常见的死亡原因。本研究旨在评价类胡萝卜素前体β-紫罗兰酮(ION)对苯并(A)芘[B(A)P]诱发的肺癌的治疗作用。B(A)P(50 mg/kg体重，每周口服两次，连续4周)诱发小鼠肺癌后，组织和血清LPO和组织标志物酶如芳烃羟基酶、γ-谷氨酰转肽酶、5‘-核苷酸酶和乳酸脱氢酶，以及血清肿瘤标志物，如癌胚抗原和神经元特异性烯醇化酶，在离子(60 mg/kg体重，每周两次，连续16周)显著降低组织酶和非酶抗氧化剂活性的情况下，观察到显著降低LPO，并将所有癌症标志物酶和抗氧化剂水平恢复到接近正常的水平这表明离子的抗癌作用。核仁组成区嗜银染色和肺组织病理分析、增殖细胞核抗原免疫组织化学和免疫印迹分析进一步证实了这一点。总体而言，结果表明，离子有效地改善了肺癌的发生，这归因于抗增殖和抗氧化的潜力，通过清除自由基的性质。
Nowadays, in developing countries like India, incidence of lung cancer is increasing rapidly, and as a consequence it has become the most common cause of malignancy-associated death. This study is aimed to evaluate the therapeutic efficacy of beta-ionone (ION), a precursor for carotenoids against benzo(a)pyrene [B(a)P]-induced lung carcinogenesis. B(a)P (50 mg/kg body weight, orally twice a week for 4 successive weeks)-induced lung cancer in mice was assessed both in tissue and serum in terms of increase LPO and tissue marker enzymes, such as aryl hydrocarbon hydroxylase, gamma-glutamyl transpeptidase, 5'-nucleotidase, and lactate dehydrogenase, and serum tumor markers such as carcinoembryonic antigen and neuron-specific enolase with concordant decrease in activities of tissue enzymic and non-enzymic antioxidants were observed on the treatment of ION (60 mg/kg body weight, orally twice a week for 16 weeks) significantly attenuated LPO and restored all cancer marker enzymes and antioxidants levels to near normal, which indicates the anticancer effect of ION. This was further confirmed by histological staining of argyrophilic nucleolar organizer region and histopathological analysis of lung tissue, immunohistochemical and immunoblot analysis of proliferating cell nuclear antigen. Overall findings suggested that the ION effectively ameliorated the lung carcinogenesis, which is attributed to the antiproliferative and antioxidant potential through free radical scavenging property.