Huqi San-Evoked Rat Colonic Anion Secretion through Increasing CFTR Expression.
Huqi San-Evoked Rat Colonic Anion Secretion through Increasing CFTR Expression.
复制标题
护气散通过增加CFTR表达诱发大鼠结肠阴离子分泌
DOI：
10.1155/2015/301640
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Xue X;Shi Z;Wang W;Yu X;Feng P;Zhang M;Wang X;Xu J
护气散(HQS)是一种由八味中草药组成的中草药制剂，具有利尿、解毒、活血、利胆等功效。转运蛋白表达和功能的缺陷会导致胆汁淤积和黄疸。然而，HQS对胆汁淤积的作用机制，尤其是对胃肠道离子分泌的影响，目前尚不清楚。采用实时荧光定量RT-PCR法和Western blotting法检测囊性纤维化跨膜电导调节因子和α-ENaC在大鼠消化道的表达和定位，并用短路电流技术观察HQS对大鼠远端结肠粘膜离子转运的影响。结果表明，HQS能显着提高肝脏和远端结肠中α-ENaC的转录和蛋白含量，但不能显着提高消化器官中CFTRmRNA的转录量和蛋白质含量。HQS增加ISC，降低跨上皮阻力。用上皮Na+通道阻滞剂预处理不影响HQS引起的ISC反应，但去除细胞外的Cl−或用Cl−通道或Na+-K+-2Cl−共转运体阻断剂预处理则可抑制HQS引起的ISC反应。这些结果表明，HQS、RA和RP可通过增加肝脏和远端结肠−的转录和蛋白含量来刺激远端结肠的ClCFTRm RNA的分泌。
Huqi San (HQS) is a Chinese herbal preparation of eight medicinal herbs that promote diuresis, detoxification, blood circulation, and cholestasis. Defects in transporter expression and function can cause cholestasis and jaundice. However, the mechanism of the cholestasis underlying HQS effects, especially on the gastrointestinal tract ion secretion, has not been elucidated. Real-time RT-PCR and Western blotting were used to study the expression and localization of cystic fibrosis transmembrane conductance regulator (CFTR) and α-ENaC in rat alimentary tract, and then the effect of HQS on the ion transport in rat distal colon mucosa was investigated using the short-circuit current (I SC) technique. The results showed that pretreatment with HQS significantly enhanced mRNA transcripts and protein content of CFTR in liver and distal colon but not α-ENaC in alimentary organs. HQS increases I SC and decreases the transepithelial resistance. Pretreatment with epithelial Na+ channel blocker did not affect the I SC responses elicited by HQS, but removal of extracellular Cl− or pretreatment with Cl− channel or Na+-K+-2Cl− cotransporter blocker inhibited HQS-elicited I SC responses. These findings demonstrated that HQS, RA, and RP can stimulate Cl− secretion in the distal colon by increasing the mRNA transcripts and protein content of CFTR in liver and distal colon.