Expression and prognostic significance of HLA class I, ICAM-1, and tumor-infiltrating lymphocytes in esophageal cancer.
Expression and prognostic significance of HLA class I, ICAM-1, and tumor-infiltrating lymphocytes in esophageal cancer.
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DOI：
10.1016/s1091-255x(97)80051-0
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发表时间：
1997-07-01
期刊：
影响因子：
--
通讯作者：
Izbicki, J R
中科院分区：
文献类型：
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作者：
Hosch, S B;Meyer, A J;Izbicki, J R
大多数实体恶性肿瘤表现出一定程度的淋巴组织浸润性，提示有一种特异性免疫宿主对肿瘤的反应。肿瘤浸润性淋巴细胞(即CD3+T淋巴细胞亚群)、人类白细胞抗原(HLA)I类分子和细胞间黏附分子-1(ICAM-1)是T细胞介导的免疫监视的关键因素。本研究旨在探讨原发性食管癌瘤内淋巴细胞的表达模式及其与人类白细胞抗原I类和细胞间黏附分子-1表达的关系，并评价其对预后的影响。有代表性的原发肿瘤标本取自55例接受根治性全块食道切除术的患者。用针对CD3的单抗对这些肿瘤的冰冻切片进行染色，以评估肿瘤浸润性淋巴细胞、人类白细胞抗原I类和ICAM-1。术后观察期5~45个月，平均19.5个月。4个肿瘤(8%)无淋巴细胞浸润，31个肿瘤(%)呈中度浸润，13个(27%)呈强浸润。24个肿瘤(45%)中有24个(45%)表达缺失。HLAI类和ICAM-1的共同表达与肿瘤的淋巴细胞浸润密切相关。卡普兰-迈耶分析显示，与无淋巴细胞浸润者相比，有淋巴细胞浸润者的无复发存活率显著提高(中位数4个月比18个月；P 18个月比7个月；P=0.0081)。目前的数据支持T细胞介导的免疫可能影响食道癌患者命运的假设。
Most solid malignancies show some degree of lymphoid infiltration suggesting a specific immunologic host vs. tumor reaction. Tumor-infiltrating lymphocytes (i.e., CD3 + T-lymphocyte subsets), the human leukocyte antigen (HLA) class I molecules, and the intercellular adhesion molecule-1 (ICAM-1) are key factors involved in T-cell-mediated immune surveillance. The present study was designed to assess the expression pattern of intratumoral lymphocyte infiltrates and their relationship to HLA class I and ICAM-1 expression with regard to primary esophageal carcinoma and to evaluate their prognostic influence. Representative samples of primary tumors were obtained from 55 patients who had undergone radical en bloc esophagectomy. Frozen sections of these tumors were stained with monoclonal antibodies directed against CD3 for the assessment of tumor-infiltrating lymphocytes, HLA class I, and ICAM-1. The mean postoperative observation period was 19.5 months (range 5 to 45 months). Lymphocyte infiltration was absent in four tumors (8%), whereas 31 tumors (64%) showed moderate and 13 (27%) showed strong infiltration. HLA class I expression was deficient in 24 tumors (45%). Coexpression of HLA class I and ICAM-1 was significantly associated with lymphocyte infiltration of the tumor. Kaplan-Meier analyses revealed a significant beneficial influence on relapse-free survival for patients with lymphocyte infiltration of primary tumors compared to those with no lymphocyte infiltration of tumors (median 4 months vs. 18 months; P 18 months vs. 7 months; P = 0.0081). The present data support the hypothesis that T-cell-mediated immunity may influence the fate of patients with esophageal cancer.