Development of a UV crosslinked biodegradable hydrogel containing adipose derived stem cells to promote vascularization for skin wounds and tissue engineering
Development of a UV crosslinked biodegradable hydrogel containing adipose derived stem cells to promote vascularization for skin wounds and tissue engineering
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DOI：
10.1016/j.biomaterials.2017.03.021
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发表时间：
2017-06-01
期刊：
影响因子：
14
通讯作者：
Hasirci, Vasif
中科院分区：
文献类型：
--
作者：
Eke, Gozde;Mangir, Naside;Hasirci, Vasif
本研究的目的是设计一种含有脂肪来源干细胞(ADSC)的真皮替代物，通过刺激快速新生血管来促进困难创面上皮肤的再生。这是通过首先合成甲基丙烯酸明胶(GeIMA)和甲基丙烯酸化透明质酸(HAMA)前体来实现的，这些前体可以在冷冻干燥后储存在-80摄氏度。然后将聚合物前体按15：1的比例溶解在介质中，ADSCs与光引发剂一起加入到40 S的W水凝胶中，在体外环境中3周内降解50%。ADSC负载的水凝胶易于用钳子夹住(压缩模数为6kpa)。凝胶的透明度将允许伤口部位的全视野。共聚焦显微镜下观察到的丝状足孔显示，水凝胶为ADSC的增殖提供了适宜的微环境。体内研究表明，与无细胞的水凝胶相比，干细胞负载的水凝胶使血管形成增加了3倍。总之，负载ADSC的GelMA/HAMA水凝胶显示出所需的增殖和血管生成特性，对于促进血管生成以促进伤口愈合和提高组织工程皮肤的存活率至关重要。(C)2017爱思唯尔有限公司。保留所有权利。
The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GeIMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 degrees C after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator and crosslinked with 40 s of W. Hydrogels degraded by 50% over 3 weeks in an in vitro environment. ADSC loaded hydrogels could be easily handled with forceps (compressive modulus was 6 kPa). Transparency of the gel would allow a full field-of-view of a wound site. The hydrogels provided a suitable microenvironment for ADSC proliferation as shown by the filopodia observed in confocal micrographs. In vivo studies demonstrated that stem cell loaded hydrogels increased vascularization by up to 3 fold compared to their cell free counterparts. In conclusion, GelMA/HAMA hydrogels loaded with ADSC showed the desired proliferative and angiogenic properties essential to promote angiogenesis for wound healing and improving survival of tissue engineered skin. (C) 2017 Elsevier Ltd. All rights reserved.