Effect of the dietary intake of fish oil on psycho-social behavioral disorder caused by social-defeat stress
Effect of the dietary intake of fish oil on psycho-social behavioral disorder caused by social-defeat stress
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DOI：
10.1016/j.physbeh.2022.113913
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发表时间：
2022-07-15
影响因子：
2.9
通讯作者：
Toda，Asuka
中科院分区：
文献类型：
--
作者：
Otsuka，Airi;Tamaya，Masaki;Toda，Asuka
暴露在心理社会压力下是导致抑郁症等人类疾病的风险因素。社会失败应激是人类心理社会应激的一个著名的啮齿动物模型，暴露于社会失败应激下的动物表现出社交回避行为。鱼油富含二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)，有望降低抑郁症的风险。在这项研究中，我们确定了鱼油是否会影响暴露于十二烷基硫酸钠的小鼠的社会行为，并测量了5-羟色胺水平和海马色氨酸(Trp)代谢相关基因的表达。实验动物在暴露于十二烷基硫酸钠期间喂食含有鱼油的日粮。对于鱼油处理，实验小鼠饲喂含有低(L-FO)、中(M-FO)和高(H-FO)鱼油的日粮。对照组补充等量的菜籽油(不含鱼油：N-FO)。在SDS方案后，我们进行了社会互动测试，并评估了实验小鼠的社会性。在N-FO组，与非应激小鼠相比，暴露于十二烷基硫酸钠的小鼠表现出负面的社会互动。L-FO组和H-FO组在抑郁暴露后表现出负性社交行为，而M-FO组没有表现出消极的社会行为。N-FO组染毒小鼠血清5-羟色胺水平低于非应激小鼠。与N-FO组的这些结果相比，在FO组中，暴露于SDS和非应激的小鼠之间的5-羟色胺水平没有差异。此外，N-FO组小鼠体内Trp代谢相关基因的表达在N-FO组增加，而FO组则无明显变化。这些结果提示，鱼油可以改善抑郁所致的心理社会行为障碍。这种改善可以用海马体中5-羟色胺合成的增加来解释。
Exposure to psychosocial stress is a risk factor for human diseases such as depression. Social defeat stress (SDS) is a well-known rodent model of human psychosocial stress, and animals exposed to SDS show social avoidance behavior. Fish oil, which is rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), is expected to decrease the risk of depressive disorders. In this study, we determined whether fish oil affects the social behavior of SDS-exposed mice and measured serotonin levels and expression of genes related to tryptophan (TRP) metabolism in the hippocampus. The experimental animals were fed a diet containing fish oil during SDS exposure. For the fish oil treatment, experimental mice were fed a diet containing fish oil at low (L-FO), middle (M-FO), and high (H-FO) concentrations. The control group was supplemented with an equivalent amount of canola oil (no fish oil: N-FO). After the SDS protocol, we performed a social interaction test and assessed the sociality of experimental mice. In the N-FO group, SDS-exposed mice showed negative social interactions compared with non-stressed mice. The L-FO and H-FO groups showed negative social interactions after SDS exposure; however, the M-FO group did not exhibit negative social behavior. The serotonin levels of SDS-exposed mice were lower than those of non-stressed mice in the N-FO group. In contrast with these results in the N-FO group, there was no difference in serotonin levels between SDS-exposed and non-stressed mice in the FO groups. In addition, the expression of genes related to TRP metabolism in SDS-exposed mice increased in the N-FO group, but not in the FO group. These results suggest that fish oil improves the psychosocial behavioral disorders caused by SDS. This improvement could be explained by the increase in serotonin synthesis in the hippocampus.