The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses
The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses
复制标题
DOI：
10.1007/s10725-016-0240-5
复制
发表时间：
2017-05-01
影响因子：
4.2
通讯作者：
Aleynova, Olga A.
中科院分区：
文献类型：
--
作者：
Dubrovina, Alexandra S.;Kiselev, Konstantin V.;Aleynova, Olga A.
钙依赖蛋白激酶（CDPKs或CPKs）是植物中钙的重要初级传感器，已知在植物非生物和生物胁迫应答中发挥重要作用。山葡萄是一种具有高度抗寒和抗病能力的野生葡萄品种。先前已表明，在盐、干旱、高甘露醇、寒冷和高温胁迫条件下，山葡萄的10个CDPK基因转录水平升高。在山葡萄植株插条中，VaCPK29的表达在高温和低温、水分亏缺以及高甘露醇胁迫下被诱导。本研究显示，与用空载体转化的对照相比，过表达VaCPK29的山葡萄愈伤组织细胞培养物以及土培的拟南芥植株对高温和高甘露醇胁迫表现出更高的耐受性。转基因山葡萄愈伤组织和拟南芥植株对寒冷、盐和干旱胁迫的耐受性与对照相当。在高温胁迫下，应激反应基因AtDREB1A、AtDREB2A、AtRD29A、AtRD29B和AtABF3在过表达VaCPK29的拟南芥植株中上调。综上所述，数据表明VaCPK29基因可能在葡萄对高温和渗透胁迫的应答中作为一个正调控因子。
Ca2+-dependent protein kinases (CDPKs or CPKs) are essential primary sensors of Ca2+ in plants and are known to play important roles in plant abiotic and biotic stress responses. Vitis amurensis is a wild grapevine species with a high level of cold and disease resistance. It has previously been shown that transcription of 10 CDPK genes of V. amurensis was elevated under salt, desiccation, high mannitol, cold, and heat stress conditions. Expression of VaCPK29 was induced under high and low temperatures, water deficit, and high mannitol stress in plant cuttings of V. amurensis. The present study revealed that the callus cell cultures of V. amurensis and soil-grown plants of Arabidopsis thaliana overexpressing VaCPK29 exhibited higher tolerance to heat and high mannitol stress in comparison with the control transformed with the empty vector. Cold, salt, and drought stress tolerance of the transgenic V. amurensis calli and A. thaliana plants was comparable to that of the controls. The stress-responsive genes AtDREB1A, AtDREB2A, AtRD29A, AtRD29B, and AtABF3 were up-regulated in the VaCPK29-overexpressing A. thaliana plants under heat stress. Taken together, the data indicate that the VaCPK29 gene may act as a positive regulator in the grapevine response to heat and osmotic stresses.