Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans: Analysis of clinical strains isolated from oral cavities
Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans: Analysis of clinical strains isolated from oral cavities
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DOI：
10.1016/j.archoralbio.2010.03.017
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
--
作者：
Taniguchi, N.;Nakano, K.;Ooshima, T.
目的：变形链球菌是龋病的主要病原体，偶尔从感染性心内膜炎患者的血液中分离到，尽管其细胞表面葡萄糖基转移酶（GTFB、GTFC和GTFD）与感染性心内膜炎的致病性的关联仍有待阐明。在这项研究中，我们研究了S。目的：研究变形链球菌GTF缺陷型同基因突变株对血小板聚集的影响，并分析其在大量临床分离株中的表达谱。使用取自小鼠的全血和富血小板血浆（PRP）以及人PRP评价变异株参考菌株MT 8148。此外，还研究了396 S.从396名受试者的口腔中分离的变形菌菌株进行了分析，通过Western印迹使用的抗血清特异性为每个GTF.Results：血小板聚集活性的GTF缺陷的同基因突变体显着低于MT 8148时，加入到大量的细胞。蛋白质印迹法显示没有菌株没有GTF表达，但6株与MT 8148相比具有GTFB和GTFC的改变。PCR分析表明，与MT 8148相比，这些菌株中的gtf 3-gtfC区域长度短约4.5kb。这些被指定为“GTFBC融合”菌株，他们表现出较低水平的血小板aggregation.Conclusions：我们的研究结果表明，GTF与血小板聚集。虽然临床检出率S.虽然具有改变的表达的变形杆菌菌株的活性极低，但GTFBC融合菌株具有与GTF缺陷型突变菌株相似的活性。(C)2010爱思唯尔有限公司版权所有。
Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates.Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF.Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PGR analyses indicated that the gtf3-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation.Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains. (C) 2010 Elsevier Ltd. All rights reserved.