Melatonin prevents obesity through modulation of gut microbiota in mice
Melatonin prevents obesity through modulation of gut microbiota in mice
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褪黑激素通过调节小鼠肠道微生物群来预防肥胖。
DOI：
10.1111/jpi.12399
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发表时间：
2017-05-01
影响因子：
10.3
通讯作者：
Zhai, Yonggong
中科院分区：
文献类型：
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作者：
Xu, Pengfei;Wang, Jialin;Zhai, Yonggong
超重和肥胖是全球范围内严重的公共健康威胁。最近的证据表明，肠道微生物区系失调是肥胖及其共病的原因之一。褪黑素的减肥和能量平衡作用已经在几项研究中得到了报道，但到目前为止，还没有关于褪黑素的有益作用是否与肠道微生物区系相关的研究。在这项研究中，我们表明褪黑素可以减轻高脂饮食(HFD)喂养的小鼠的体重、肝脏脂肪变性和低度炎症，并改善胰岛素抵抗。对16S rRNA的高通量焦解测序表明，褪黑素治疗显著改变了喂食HFD的小鼠肠道微生物区系的组成。褪黑素显著降低了肠道微生物区系的丰富度和多样性。与正常饮食(NCD)组相比，高脂饲料组改变了69个操作分类单位(OTU)，补充褪黑素将14个OTU逆转为与NCD组相同的结构，从而影响了各种功能，特别是通过其降低Firmicut-Bacteroidtes比率和增加粘蛋白降解菌Akkermansia的丰度的能力，这与健康黏膜有关。综上所述，我们的结果表明褪黑素可能被用作一种益生菌制剂来逆转HFD诱导的肠道微生物区系失调，并帮助我们更好地理解褪黑激素的各种有益作用的机制。
Excess weight and obesity are severe public health threats worldwide. Recent evidence demonstrates that gut microbiota dysbiosis contributes to obesity and its comorbidities. The body weight-reducing and energy balancing effects of melatonin have been reported in several studies, but to date, no investigations toward examining whether the beneficial effects of melatonin are associated with gut microbiota have been carried out. In this study, we show that melatonin reduces body weight, liver steatosis, and low-grade inflammation as well as improving insulin resistance in high fat diet (HFD)-fed mice. High-throughput pyrosequencing of the 16S rRNA demonstrated that melatonin treatment significantly changed the composition of the gut microbiota in mice fed an HFD. The richness and diversity of gut microbiota were notably decreased by melatonin. HFD feeding altered 69 operational taxonomic units (OTUs) compare with a normal chow diet (NCD) group, and melatonin supplementation reversed 14 OTUs to the same configuration than those present in the NCD group, thereby impacting various functions, in particular through its ability to decrease the Firmicutes-to-Bacteroidetes ratio and increase the abundance of mucin-degrading bacteria Akkermansia, which is associated with healthy mucosa. Taken together, our results suggest that melatonin may be used as a probiotic agent to reverse HFD-induced gut microbiota dysbiosis and help us to gain a better understanding of the mechanisms governing the various melatonin beneficial effects.