Cadherin-related neuronal receptors in incisor development
Cadherin-related neuronal receptors in incisor development
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DOI：
10.1177/154405910308200105
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发表时间：
2003-01-01
影响因子：
7.6
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Fukumoto, E;Sakai, H;Kato, Y
钙粘蛋白是对组织发育至关重要的细胞粘附分子。在这份报告中，我们确定了钙粘蛋白家族钙粘蛋白相关的神经元受体（CNRs）1和5在大鼠切牙表达的差异显示方法的成员。定量RT-PCR结果显示，CNR 1 mRNA在分泌期表达，但在成熟早期表达减少，而CNR 5 mRNA在这两个阶段均有表达。原位杂交结果表明，CNR 1在分泌期表达较强，在成熟早期表达减弱，成熟后期表达减弱。CNR 5 mRNA在分泌期和早熟期的早期阶段几乎以相同的水平表达，但在早熟期的晚期阶段不表达。CNR 1和5 mRNA在成牙本质细胞中持续表达。免疫组化结果显示，CNR蛋白在成釉细胞的分泌期和早期成熟期均有表达，而在晚期成熟期未见表达。CNR蛋白在成牙本质细胞中持续表达。我们发现，重组CNR 1结合牙上皮细胞和间充质细胞通过N端结构域EC 1在体外。这些结果表明CNR I和CNR 5可能在釉质和牙本质形成中起重要作用，可能通过细胞-细胞和/或细胞-基质相互作用。
Cadherins are cell adhesion molecules that are critical for tissue development. In this report, we identified members of the cadherin family cadherin-related neuronal receptors (CNRs) 1 and 5 expressed in rat incisors by the differential display method. Quantitative RT-PCR revealed that CNR1 mRNA is expressed in the secretory stage but reduced in the early-maturation stage, while CNR5 mRNA is expressed in both these stages. In situ hybridization showed that strong expression of CNR1 is strong in the secretory stage, but reduced in the early phase and diminished in the late phase of the early-maturation stage. CNR5 mRNA is expressed almost at the same levels in the secretory and in the early phase of the early-maturation stages but is absent in the late phase of the early-maturation stage. Both CNR1 and 5 mRNA are continuously expressed in odontoblasts. Immunohistology showed that CNR proteins are expressed in the secretory and early-maturation stages of ameloblasts, but no protein expression at the late-maturation stage was observed. CNR proteins were continuously expressed in odontoblasts. We found that recombinant CNR1 binds dental epithelial and mesenchymal cells through N-terminal domain EC1 in vitro. These results suggest that CNR I and CNR5 may play an important role in enamel and dentin formation, probably through cell-cell and/or cell-matrix interactions.