CHEMILUMINESCENCE DETECTION USING REGENERABLE TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) IMMOBILIZED IN NAFION
CHEMILUMINESCENCE DETECTION USING REGENERABLE TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) IMMOBILIZED IN NAFION
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DOI：
10.1021/ac00027a005
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发表时间：
1992-02-01
影响因子：
7.4
通讯作者：
NIEMAN, TA
中科院分区：
文献类型：
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作者：
DOWNEY, TM;NIEMAN, TA
研究了三（2，2 '-联吡啶）钌（Ru（bpy）3（2+））在Nation膜电极上的电化学发光检测方法。 电极电位的控制控制反应试剂Ru（bpy）3（3+）的产生，其与某些分析物反应以产生强度与分析物浓度成比例的化学发光发射。 反应导致Ru（bpy）3（3+）转化为Ru（bpy）3（2+），然后在电极处再次循环为Ru（bpy）3（3+）。 该传感器已被用于流动注射，以确定草酸，烷基胺，和NADH。 检测限分别为1 μ M、10 nM和1 μ M，工作范围超过40倍浓度。 灵敏度在3至10的pH范围内保持恒定。 与草酸盐，并在很小程度上与胺，发射强度随着离子强度的增加而增加，这被证明是一种现象，与Nafion膜，而不是化学发光反应。 发射强度随温度升高而增加。 传感器在适宜的储存条件下可保持稳定数天。 大量的Ru（bpy）3（3+）被证明能够存储在膜中。
The development of a detection method based on the electrogenerated chemiluminescence of tris(2,2'-bipyridine)ruthenium(II), (Ru(bpy)3(2+)), immobilized in a Nation film coated on an electrode is discussed. Control of the electrode potential controls creation of the reactive reagent Ru(bpy)3(3+) which reacts with certain analytes to yield chemiluminescence emission of intensity proportional to the analyte concentration. The reaction results in Ru(bpy)3(3+) being converted to Ru(bpy)3(2+), which then is recycled to Ru(bpy)3(3+) again at the electrode. This sensor has been used in flow injection to determine oxalate, alkylamines, and NADH. Detection limits are 1-mu-M, 10 nM, and 1-mu-M, respectively, with working ranges extending over 4 decades in concentration. Sensitivity is constant over the wide pH range from 3 to 10. With oxalate, and to a small extent with amines, emission intensities increase with increasing lonic strength; this was shown to be a phenomenon related to the Nafion film and not to the chemlluminescence reaction. Emission intensities increase with temperature. The sensor remains stable for several days with sultable storage conditions. Significant amounts of Ru(bpy)3(3+) are shown to be capable of storage within the film.