Isolation and characterization of a cDNA clone for the complete protein coding region of the 6 subunit of the mouse acetylcholine receptor
Isolation and characterization of a cDNA clone for the complete protein coding region of the 6 subunit of the mouse acetylcholine receptor
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小鼠乙酰胆碱受体 6 亚基完整蛋白编码区 cDNA 克隆的分离和表征
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通讯作者：
Norman Davidson
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作者：
R. LaPolla;K. Mixter;MAYNEt;Norman Davidson
已分离出一个小鼠cDNA克隆，其含有与电鳐乙酰胆碱受体（AcChoR）的8亚基高度同源的蛋白质的完整编码区。利用来自BC 3 H-1小鼠细胞的膜相关poly（A）+ RNA在载体XgtlO中构建了cDNA文库。令人惊讶的是，通过与编码电鳐Ac-ChoR γ亚基的cDNA杂交选择了8克隆.小鼠cDNA克隆的核苷酸序列含有520个氨基酸的开放阅读框。该氨基酸序列分别与鱼雷AcChoR 8和y亚基具有59%和50%的序列同源性。然而，小鼠的核苷酸序列有几个高度同源性的鱼雷y亚基cDNA的延伸，但不与8。小鼠蛋白质具有与鱼雷亚基相同的一般结构特征。它由3.3-脱氢酶mRNA编码。可能只有一个，但最多两个，染色体基因编码这个或密切相关的序列。
A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the 8 subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector XgtlO from membrane-associated poly(A)+ RNA from BC3H-1 mouse cells. Surprisingly, the 8 clone was selected by hybridization with cDNA encoding the y subunit of the Torpedo Ac- ChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR 8 and y subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo y subunit cDNA, but not with 8. The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.