The transcription/migration interface in heart precursors of Ciona intestinalis
The transcription/migration interface in heart precursors of Ciona intestinalis
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DOI：
10.1126/science.1158170
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发表时间：
2008-06-06
期刊：
影响因子：
56.9
通讯作者：
Levine, Michael
中科院分区：
文献类型：
--
作者：
Christiaen, Lionel;Davidson, Brad;Levine, Michael
基因调控网络指导胚胎发生过程中细胞命运的逐步决定，但它们如何控制形态发生过程中的细胞行为仍然很难理解。细胞分选，微阵列，和有针对性的分子操作被用来分析心脏细胞迁移海鞘。心脏网络调节涉及迁移所需的大多数细胞活动的基因，包括粘附，细胞极性和膜突起。我们证明成纤维细胞生长因子信号传导和叉头转录因子FoxF直接上调小的鸟苷三磷酸酶RhoDF，其与Cdc42协同作用以促进迁移细胞的增殖活性。此外，RhoDF诱导膜突起独立于迁移所需的其他细胞活动。我们建议，特定的效应基因的转录调控决定了离散的细胞迁移模块的协调部署。
Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.