Prevalence and characterization of Staphylococcus aureus isolated from goat milk powder processing plants
Prevalence and characterization of Staphylococcus aureus isolated from goat milk powder processing plants
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羊奶粉加工厂分离出的金黄色葡萄球菌的流行情况和特征
DOI：
10.1016/j.foodcont.2015.06.042
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发表时间：
2016-01-01
期刊：
影响因子：
6
通讯作者：
Wu, Congming
中科院分区：
文献类型：
--
作者：
Xing, Xiaonan;Zhang, Yang;Wu, Congming
金黄色葡萄球菌是食品加工业的主要关注点，因为它的毒力因子。本研究的目的是确定S。对羊奶粉加工厂分离的金黄色葡萄球菌进行了药敏试验、脉冲场凝胶电泳（PFGE）和PCR检测。PCR检测葡萄球菌肠毒素（sea to set）、葡萄球菌肠毒素样（self to selv）、中毒性休克综合征毒素-1（tst）、剥脱毒素（eta和etb）、Paton-Valentine杀白细胞素（pvl）和甲氧西林耐药（mecA）基因。共采集910个样品，其中62个来自挤奶站的生羊奶样品和848个来自4个羊奶粉厂的7个不同采样点的样品。在910份样品中，95份（10.4%）样品，包括62份挤奶站样品中的34份（54.8%）和848份奶粉加工厂样品中的61份（7.2%）样品中的S阳性。金黄色。63.2%的菌株含有一个或多个毒力基因。5个主要毒力基因依次为pv/（29.5%）、sec（23.2%）、ser（16.8%）、tst（14.7%）和seb（12.6%）。90.5%的菌株对至少一种抗生素耐药。最常见的耐药药物为甲氧苄啶/磺胺甲恶唑（89.5%）、红霉素（30.5%）、四环素（22.1%）、氨苄西林（16.8%）、氯霉素（15.8%）和利福平（9.5%）。所有菌株共产生44种PFGE图谱。PFGE结果表明，部分具有相同PFGE图谱的分离株来自羊奶粉不同加工阶段。我们的研究结果表明，S。金黄色葡萄球菌在不同的羊奶粉产地之间传播。此外，S.金黄色葡萄球菌存在于羊奶粉加工阶段。S.金黄色葡萄球菌菌株在羊奶粉加工阶段对公众健康构成潜在威胁。(C)2015爱思唯尔有限公司版权所有。
Staphylococcus aureus is a major concern for the food processing industry because of its virulence factors. The aim of this study was to determine the prevalence of S. aureus strains in goat milk powder processing plants, and to characterize these strains by antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE) and PCR. PCR detected genes encoding staphylococcal enterotoxin (sea to set), staphylococcal enterotoxin-like (self to selv), toxic-shock syndrome toxin-1 (tst), exfoliative toxin (eta and etb), Paton-Valentine Leukocidin (pvl), and methicillin resistance (mecA). A total of 910 samples including 62 raw goat milk samples from a milking station and 848 samples from seven different sampling sites in four goat milk powder plants were collected. Out of 910 samples, 95 (10.4%) samples, including 34 (54.8%) of 62 milking station samples and 61(7.2%) of 848 milk powder processing plant samples, were positive for S. aureus. 63.2% of 95 isolates contained one or more virulence genes. The five most predominant virulence genes were pv/ (29.5%), sec (23.2%), ser (16.8%), tst (14.7%), and seb (12.6%). 90.5% of strains were resistant to at least one antibiotic. Resistance was most frequently observed to trimethoprim/sulfamethoxazole (89.5%), erythromycin (30.5%), tetracycline (22.1%), ampicillin (16.8%), chloramphenicol (15.8%), and rifampicin (9.5%). A total of 44 PFGE patterns were generated among all the isolates. PFGE demonstrated that some isolates with the same PFGE patterns came from different goat milk powder processing stages. Our findings indicate that S. aureus has spread among different goat milk powder producing locations. In addition, cross-contamination of S. aureus exists in goat milk powder processing stages. The presence of S. aureus strains in goat milk powder processing stages poses a potential threat to public health. (C) 2015 Elsevier Ltd. All rights reserved.