Phytochemical Contents of Different Parts of the Seeded Raisins from the South-East Anatolia: Enzyme Inhibitory Potential of Pulp Extracts
Phytochemical Contents of Different Parts of the Seeded Raisins from the South-East Anatolia: Enzyme Inhibitory Potential of Pulp Extracts
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DOI：
10.1002/cbdv.202100844
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发表时间：
2022-01-27
影响因子：
2.9
通讯作者：
Ertas, Abdulselam
中科院分区：
文献类型：
--
作者：
Karatas, Dilek Degirmenci;Oz, Veysi;Ertas, Abdulselam
在这项研究中，一些植物化学性质的种子葡萄干，主要是在东南安纳托利亚种植。此外，一些物理和质量特性，酚含量（通过LC-MS/MS;液相色谱串联质谱/质谱仪系统）、抗胆碱酯酶和抗氧化能力（DPPH; 2，2-二苯基-1-苦基肼基自由基清除活性，ABTS; 2，2-连氮基双（3-乙基苯并噻唑啉-6-磺酸）阳离子自由基清除活性和CUPRAC;铜还原抗氧化能力）的品种进行了调查。在所有三种方法中，种子提取物的抗氧化活性值被确定为高于叶和果肉提取物。值得注意的是，Banazi Siyahi的种子提取物在ABTS（IC 50：4.35 +/- 0.02 μ g/mL）、DPPH（IC 50：10.78 +/- 0.78 μ g/mL）和CUPRAC（A0.5：9.33 +/- 0.45 μ g/mL）方法中显示出最高的抗氧化活性。此外，所有纸浆样品的乙醇提取物对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）酶比加兰他敏表现出更高的抗胆碱酯酶活性。根据LC-MS/MS结果，发现Kerkus种子提取物中含有丰富的儿茶素（21.362 mg分析物/g提取物）和表儿茶素（44.667 mg分析物/g提取物），异槲皮苷（116.873 mg分析物/g提取物）和黄芪苷（31.915 mg分析物/g提取物）检测到Banazi Siyahi叶提取物中含量相当丰富。考虑到葡萄品种和它们生长的土壤样本的矿物质含量，研究中分析的所有葡萄品种都被发现是丰富的。基于这些发现，可能表明Banazi Siyahi和Kerkus品种具有潜在的利用在制药和食品工业，由于其儿茶素，异槲皮苷和甘草酸的含量。
In this study, some phytochemical properties of six seeded raisin species that are mainly cultivated in Southeastern Anatolia were investigated. Additionally, some physical and quality characteristics, phenolic contents (by LC-MS/MS; Liquid Chromatography Tandem Mass/Mass Spectrometer System), anticholinesterase, and antioxidant capacities (DPPH; 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging, ABTS; 2,2-azinobis(3-ethylbenzothiazoline-6-sulphonic acid cation-radical scavenging activity and CUPRAC; cupric reducing antioxidant capacity) of the cultivars were investigated on ground raisins. In all three methods, the antioxidant activity values of seed extracts were determined to be higher than those of leaf and pulp extracts. Remarkably, the seed extract of Banazi Siyahi showed the highest antioxidant activity in ABTS (IC50: 4.35 +/- 0.02 mu g/mL), DPPH (IC50: 10.78 +/- 0.78 mu g/mL), and CUPRAC (A0.5: 9.33 +/- 0.45 mu g/mL) methods. Additionally, the ethanol extracts of all pulp samples showed higher anticholinesterase activity against acetyl-(AChE) and butyrylcholinesterase (BChE) enzymes than galantamine. According to the LC-MS/MS results, catechin (21.362 mg analyte/g extract) and epicatechin (44.667 mg analyte/g extract) found to be quite rich in Kerkus seed extract and isoquercitrin (116.873 mg analyte/g extract) and astragalin (31.915 mg analyte/g extract) detected to be quite rich in Banazi Siyahi leaf extract. Considering the mineral content of the varieties and the soil samples they grow in, all of the grape varieties analyzed in the study was found to be rich. Based on these findings, it might be suggested that Banazi Siyahi and Kerkus varieties have potential to be utilized in pharmaceutical and food industries, due to their contents of catechins, isoquercitrin and astragalin.