Paramagnetic relaxation-based 19F MRI probe to detect protease activity
Paramagnetic relaxation-based 19F MRI probe to detect protease activity
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DOI：
10.1021/ja077058z
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发表时间：
2008-01-23
影响因子：
15
通讯作者：
Kikuchi, Kazuya
中科院分区：
文献类型：
--
作者：
Mizukami, Shin;Takikawa, Rika;Kikuchi, Kazuya
提出了一种新的F-19型核磁共振检测蛋白水解酶探针的设计原理。这一原理基于Gd3+分子内顺磁效应对F-19磁共振信号的猝灭。分子内Gd3+显著地减弱了F-19探针信号，caspase-3对探针的水解作用抵消了顺磁效应。使用该探针，该探针可以从F-19磁共振成像的模型图像中检测到caspase-3的空间活性。
A novel design principle for F-19 MRI probes detecting protease activity was developed. This principle is based on F-19 MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the F-19 probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using F-19 MRI.