Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
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DOI：
10.1084/jem.177.4.897
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发表时间：
1993-04-01
影响因子：
15.3
通讯作者：
Pals, S T
中科院分区：
文献类型：
--
作者：
Koopman, G;Heider, K H;Horst, E;Adolf, G R;van den Berg, F;Ponta, H;Herrlich, P;Pals, S T
最近描述的剪接变异体的CD44表达在转移细胞系的大鼠肿瘤，已被证明赋予转移潜力的非转移大鼠胰腺癌和肉瘤细胞系。使用针对由变体CD44序列编码的细菌融合蛋白的抗体，我们已经探索了变体CD44糖蛋白在人淋巴细胞和组织以及非霍奇金淋巴瘤上的表达。正常的淋巴造血细胞表达几乎检测不到的低水平的变体CD44糖蛋白，而T淋巴细胞，在有丝分裂原或抗原激活后，瞬时上调特异性CD44变体糖蛋白的表达。各种抗体的反应模式表明，这些CD44变体含有外显子v6编码的结构域，其是在大鼠中赋予转移能力的变体的一部分。有趣的是，v6的过表达也在几种侵袭性非霍奇金淋巴瘤中发现，但在低级别非霍奇金淋巴瘤中没有发现。
A recently described splice variant of CD44 expressed in metastasizing cell lines of rat tumors, has been shown to confer metastatic potential to nonmetastasizing rat pancreatic carcinoma and sarcoma cell lines. Using antibodies raised against a bacterial fusion protein encoded by variant CD44 sequences, we have explored the expression of variant CD44 glycoproteins on human lymphoid cells and tissues and on non-Hodgkin's lymphomas. Normal lymphohematopoietic cells express barely detectable low levels of variant CD44 glycoproteins, whereas T lymphocytes, upon activation by mitogen or antigen, transiently upregulate expression of specific CD44 variant glycoproteins. The reaction pattern of various antibodies indicates that these CD44 variants contain the domain encoded by exon v6, which is part of the variant that in the rat confers metastatic capability. It is interesting that overexpression of v6 was also found in several aggressive, but not low-grade, non- Hodgkin's lymphomas.