Down-Regulation of Stat3 Decreases Invasion Activity and Induces Apoptosis of Human Glioma Cells
Down-Regulation of Stat3 Decreases Invasion Activity and Induces Apoptosis of Human Glioma Cells
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DOI：
10.1007/s12031-009-9323-3
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发表时间：
2010-03-01
影响因子：
3.1
通讯作者：
Ji, Yonghua
中科院分区：
文献类型：
--
作者：
Chen, Fuxue;Xu, Yaming;Ji, Yonghua
胶质瘤是最常见的原发性脑肿瘤。尽管目前胶质瘤的治疗方法有所改善，包括手术切除、放疗和化疗，但在治疗这类疾病方面进展甚微。Stat3是转录家族的信号换能器和激活子。它在调节细胞存活、侵袭和凋亡中起重要作用。本研究探讨Stat3低水平表达对U251细胞侵袭和凋亡的影响。我们的数据显示Stat3在人胶质瘤细胞系U251中组成性表达。下调Stat3后，U251细胞的侵袭活性减弱，诱导U251细胞凋亡。此外，下调Stat3可抑制MMP-2、Bcl-xL和survivin的表达，但不抑制67LR的表达。这些结果进一步表明Stat3在人胶质瘤细胞系U251的侵袭和凋亡中起关键作用。
Gliomas are the most common type of primary brain tumors. Despite the improvement in current treatments for gliomas, including surgical resection, radiation, and chemotherapy, there has been very little progress in curing this kind of disease. Stat3 is a member of signal transducer and activator of transcription family. It plays an important role in regulating cell survival, invasion, and apoptosis. This study investigated the influence of low-level expression of Stat3 on invasion and apoptosis in U251 cells. Our data showed that Stat3 is constitutively expressed in human gliomas cell line U251. The invasion activity in U251 cells was weakened and the apoptosis in U251 cells was induced after down-regulation of Stat3. In addition, down-regulation of Stat3 can suppress the expression of MMP-2, Bcl-xL and survivin but not 67LR. These results further indicate that Stat3 plays a key role in the invasion and apoptosis of human glioma cell line U251.