Comparison of Genome-wide Expression Patterns in Response to Heavy Metal Treatment in Saccharomyces cerevisiae
Comparison of Genome-wide Expression Patterns in Response to Heavy Metal Treatment in Saccharomyces cerevisiae
复制标题
酿酒酵母重金属处理响应的全基因组表达模式比较
DOI：
10.1273/cbij.1.41
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发表时间：
2001
影响因子：
0.3
通讯作者：
H. Iwahashi
中科院分区：
文献类型：
--
作者：
Y. Momose;E. Kitagawa;H. Iwahashi
cDNA 微阵列是非常方便的工具，可用于了解全基因组基因表达模式的变化。酵母DNA微阵列在载玻片上包含5880个ORF cDNA探针，几乎是整个基因组。我们使用酵母 DNA 微阵列分析了暴露于镉 (Cd) 或汞 (Hg) 后的全基因组转录谱，虽然两种重金属诱导了 22 个基因，但这些谱存在许多差异。从这些结果中，我们得出结论，酵母微阵列对于环境因素的生物测定很有价值。
cDNA microarrays are very convenient tools that can be used to understand changes in genome-wide patterns of gene expression. The yeast DNA microarray contains 5880 ORF cDNA probes, which is almost whole genome, on a glass slide. We analyzed genome-wide transcript profiles following exposure to Cadmium (Cd) or Mercury (Hg) with the yeast DNA microarray, and while 22 genes were induced by both heavy metals, there were many discrepancies in those profiles. From these results, we concluded that yeast microarrays are valuable for bioassay of environmental agents.