Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma.
Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma.
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胶质瘤中癌/睾丸抗原OY-TES-1的血清免疫反应性及其组织表达
DOI：
10.3892/ol.2017.5799
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发表时间：
2017-05
期刊：
影响因子：
2.9
通讯作者：
Xiao S
中科院分区：
文献类型：
--
作者：
Li X;Yan J;Fan R;Luo B;Zhang Q;Lin Y;Zhou S;Luo G;Xie X;Xiao S
OY - TES - 1是癌/睾丸抗原家族的成员，在健康的睾丸组织和某些类型的癌组织中表达。本研究旨在分析脑胶质瘤患者中OY - TES - 1的表达模式以及血清抗OY - TES - 1抗体浓度。通过常规逆转录聚合酶链反应（RT - PCR）分析，在36例脑胶质瘤病例中的28例（78%）检测到OY - TES - 1 mRNA。实时定量PCR显示，与正常成人组织（睾丸组织除外）相比，OY - TES - 1在脑胶质瘤组织中表达水平更高。在36例脑胶质瘤患者中有5例（14%）的血清中存在抗OY - TES - 1抗体，但在107例健康供体的所有血清样本中均未检测到。免疫组化分析表明，在抗OY - TES - 1抗体血清阳性患者的所有脑胶质瘤组织中均表达OY - TES - 1蛋白。这些结果表明，OY - TES - 1是脑胶质瘤免疫治疗的一个新的候选靶点。
OY-TES-1 is a member of the cancer/testis antigen family that is expressed in healthy testis tissue and certain types of cancerous tissue. The present study aimed to analyze the expression pattern of OY-TES-1 and serum anti-OY-TES-1 antibody concentration in patients with glioma. OY-TES-1 mRNA was detected in 28/36 (78%) of glioma cases using conventional reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-quantitative-PCR revealed that OY-TES-1 was expressed at a higher level in glioma tissues compared with normal adult tissues (with the exception of testis tissue). Anti-OY-TES-1 antibodies were present in the serum of 5/36 (14%) of patients with glioma, but absent in all the serum samples from 107 healthy donors. Immunohistochemical analysis demonstrated that OY-TES-1 protein was expressed in all glioma tissues from patients with anti-OY-TES-1 antibody seropositivity. These results suggest that OY-TES-1 is a novel candidate for glioma immunotherapy.