Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase
Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase
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DOI：
10.1016/j.ab.2006.06.017
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发表时间：
2006-09-01
影响因子：
2.9
通讯作者：
Harris, Robert A.
中科院分区：
文献类型：
--
作者：
Jeoung, Nam Ho;Sanghani, Paresh C.;Harris, Robert A.
丙酮酸脱氢酶复合物的活性长期以来在一些实验室中通过将乙酰辅酶A（乙酰辅酶A）的产生与4-氨基偶氮苯-4-磺酸通过芳胺N-乙酰转移酶的乙酰化偶联来测定。该测定法具有一些优点，但其使用受到需要大量芳胺N-乙酰转移酶的限制。在这里，我们报告生产重组鸡肝芳胺N-乙酰转移酶和优化其使用的小型化丙酮酸脱氢酶复合物及其激酶的测定。(c)2006年爱思唯尔公司All rights reserved.
The activity of the pyruvate dehydrogenase complex has long been determined in some laboratories by coupling the production of acetyl-coenzyme A (acetyl-CoA) to the acetylation of 4-aminoazobenzene-4-sulfonic acid by arylamine N-acetyltransferase. The assay has some advantages, but its use has been limited by the need for large amounts of arylamine N-acetyltransferase. Here we report production of recombinant chicken liver arylamine N-acetyltransferase and optimization of its use in miniaturized assays for the pyruvate dehydrogenase complex and its kinase. (c) 2006 Elsevier Inc. All rights reserved.