Desiccation and rehydration elicit distinct heat shock protein transcript responses in flesh fly pupae
Desiccation and rehydration elicit distinct heat shock protein transcript responses in flesh fly pupae
复制标题
DOI：
10.1242/jeb.00842
复制
发表时间：
2004-02-01
影响因子：
2.8
通讯作者：
Denlinger, DL
中科院分区：
文献类型：
--
作者：
Hayward, SAL;Rinehart, JP;Denlinger, DL
热休克蛋白（Hsps）是原核生物和真核生物对细胞应激反应中普遍存在的成分，但它们在陆生节肢动物干燥应激中的作用和功能受到的关注有限。在维持细胞完整性和酶活性方面，对再水化的分子反应可以说与对干燥的反应一样重要，但热休克蛋白在应激恢复中的作用知之甚少，并且从未在昆虫的再水化方面得到解决。本研究发现了肉蝇Sarcophaga crassipalpis对脱水和复水化的热休克反应存在明显差异，以及滞育蛹和非滞育蛹的干燥反应存在差异。在非滞育蛹中，两种可诱导热休克蛋白（Hsp23和Hsp70）的表达因脱水而上调，但热休克蛋白表达的失水阈值在不同脱水速率下发生变化。持续干燥导致Hsp23和Hsp70的表达延长，这可能导致在干燥超过3天的样品中观察到的成虫羽化延迟
Heat shock proteins (Hsps) are a ubiquitous component of the cellular response to stress in both prokaryotic and eukaryotic organisms, but their role and function during desiccation stress in terrestrial arthropods has received limited attention. Molecular responses to rehydration are arguably as important as those to desiccation in maintaining cellular integrity and enzyme activity, but the role of Hsps during stress recovery is poorly understood and has never been addressed with respect to rehydration in insects. This study identifies distinct differences in the Hsp response to desiccation and rehydration in the flesh fly Sarcophaga crassipalpis, as well as differences in the desiccation responses of diapausing and nondiapausing pupae. In nondiapausing pupae, the expression of two inducible Hsps (Hsp23 and Hsp70) is upregulated by desiccation, but the water loss threshold for Hsp expression changes at different rates of dehydration. Continued desiccation results in the prolonged expression of both Hsp23 and Hsp70, which may contribute to the delayed adult eclosion noted in samples desiccated for more than 3 days at