Characterization of peanut-lectin (+) cells derived from the RCCT-28A cell line.
Characterization of peanut-lectin (+) cells derived from the RCCT-28A cell line.
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源自 RCCT-28A 细胞系的花生凝集素 ( ) 细胞的表征。
DOI：
10.1038/ki.1993.30
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发表时间：
1993
影响因子：
19.6
通讯作者：
Bello-Reuss,E
中科院分区：
文献类型：
--
作者：
Bello-Reuss,E
源自 RCCT-28A 细胞系的花生凝集素 (+) 细胞的表征。分离并培养具有最高花生凝集素结合能力的 RCCT-28A 细胞系的细胞 [RCCT-28A(P+)]，试图获得 HCO3 分泌细胞群。研究了汇合单层中顶端溶液酸或碱挤出的机制。在 RCCT-28A (P+) 细胞中，净 H+ 通量 (JH+, nmol·min-1·cm-2) 显着低于 (JH+ = 13 ± 3) 在 RCCT-28A 细胞 (21 ± 2) 中观察到的值。 N-乙基马来酰亚胺、巴弗洛霉素-A、奥美拉唑和先灵 28080 降低 JH+。无论顶侧存在或不存在 SCH，从基底外侧去除 Cl- 都会降低 JH+。去除顶端Cl-取消了碱基等价物的顶端挤出。在高碳酸氢盐溶液中孵育会导致顶端侧产生碱当量通量 (JH+ = -7.0 ± 1.0)。在 pH 7.5 的高渗介质（添加甘露醇）中预孵育可将 JH+ 降低至 -3.0 ± 1.0。我们的结论是，在功能上，RCCT-28A(P+) 细胞与 RCCT-28A 细胞仅在数量上有所不同。这些细胞的酸分泌可以通过碱性和高渗预孵育来调节，并且很可能是由顶端 H+-ATP 酶和 H+,K+-ATP 酶的共存介导的。净 OH 分泌的主要机制似乎是顶端侧的 Cl-/HCO3-交换。
Characterization of peanut-lectin (+) cells derived from the RCCT-28A cell line. Cells from RCCT-28A cell line, which exhibited the highest peanut-lectin binding capacity [RCCT-28A(P+)] were separated and cultured attempting to obtain a population of HCO3-secreting cells. The mechanisms of apical solution acid or base extrusion were studied in confluent monolayers. In RCCT-28A (P+) cells net H+flux (JH+, nmol · min-1· cm-2) was significantly lower (JH+ = 13 ± 3) than that observed in the RCCT-28A cells (21 ± 2). N-ethylmaleimide, Bafilomycin-A, omeprazole and Schering 28080 decreased JH+. Cl-removal from the basolateral side, in the presence or absence of SCH in the apical side, decreased JH+. Removal of apical Cl-abolished apical extrusion of base-equivalents. Incubation in a high bicarbonate solution results in base-equivalent flux to the apical side (JH+ = -7.0 ± 1.0). Preincubation in a hyperosmotic medium (mannitol addition) at pH 7.5 decreased JH+ to -3.0 ± 1.0. We conclude that, functionally, RCCT-28A(P+) cells are only quantitatively different from RCCT-28A cells. Acid secretion by these cells can be modulated by alkaline and by hyperosmolar preincubation and is most likely mediated by coexistent of apical H+-ATPase and H+,K+-ATPase. The main mechanism of net OH-secretion appears to be Cl-/HCO3-exchange at the apical side.