Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.
Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.
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DOI：
10.1038/s41477-023-01447-4
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发表时间：
2023-07
期刊：
影响因子：
18
通讯作者：
Jones, Alexander M.
中科院分区：
文献类型：
--
作者：
Rowe, James;Grange-Guermente, Mathieu;Exposito-Rodriguez, Marino;Wimalasekera, Rinukshi;Lenz, Martin O.;Shetty, Kartika N.;Cutler, Sean R.;Jones, Alexander M.
植物激素脱落酸（ABA）在非生物胁迫下积累，重塑水分关系和发育。为了克服高分辨率敏感报告的缺乏，我们开发了abacus2s -下一代Förster共振能量转移（FRET）生物传感器，具有高亲和力，信噪比和正交性，可以揭示拟南芥中内源ABA的模式。我们绘制了高分辨率的应激诱导ABA动态图，以揭示局部和系统ABA功能的细胞基础。叶片湿度降低时，根细胞在韧皮部转运ABA卸载的伸长区积累ABA。韧皮部ABA和根系ABA信号都是维持低湿条件下根系生长所必需的。ABA协调根系对叶面胁迫的响应，使植物能够维持深层土壤的觅食以吸收水分。ABACUS2 Förster共振能量转移生物传感器允许在活的植物脱落酸积累和消耗的无与伦比的视图。水分充足的根在茎部脱水时在生长细胞中积累脱落酸，这是在低湿度条件下维持根生长所必需的。
The plant hormone abscisic acid (ABA) accumulates under abiotic stress to recast water relations and development. To overcome a lack of high-resolution sensitive reporters, we developed ABACUS2s—next-generation Förster resonance energy transfer (FRET) biosensors for ABA with high affinity, signal-to-noise ratio and orthogonality—that reveal endogenous ABA patterns in Arabidopsis thaliana. We mapped stress-induced ABA dynamics in high resolution to reveal the cellular basis for local and systemic ABA functions. At reduced foliar humidity, root cells accumulated ABA in the elongation zone, the site of phloem-transported ABA unloading. Phloem ABA and root ABA signalling were both essential to maintain root growth at low humidity. ABA coordinates a root response to foliar stresses, enabling plants to maintain foraging of deeper soil for water uptake. ABACUS2 Förster resonance energy transfer biosensors allow an unparalleled view of abscisic acid accumulations and depletions in living plants. Well-watered roots accumulate abscisic acid in growing cells upon shoot dehydration and this is essential to maintain root growth under low humidity.
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影响因子：
3.8
作者：
Inomata, M;Hirai, N;Ohigashi, H
通讯作者：
Ohigashi, H
影响因子：
7.4
作者：
Kuromori, Takashi;Sugimoto, Eriko;Shinozaki, Kazuo
通讯作者：
Shinozaki, Kazuo
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
2.8
作者：
Ikegami, Keiichi;Okamoto, Masanori;Koshiba, Tomokazu
通讯作者：
Koshiba, Tomokazu
DOI：
10.1111/tpj.15067
发表时间：
2021-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Hsu PK;Dubeaux G;Takahashi Y;Schroeder JI
通讯作者：
Schroeder JI