Developmental role of dpp in the gastropod shell plate and co-option of the dpp signaling pathway in the evolution of the operculum
Developmental role of dpp in the gastropod shell plate and co-option of the dpp signaling pathway in the evolution of the operculum
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DOI：
10.1016/j.ydbio.2012.04.010
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发表时间：
2012-06-15
影响因子：
2.7
通讯作者：
Wada, Hiroshi
中科院分区：
文献类型：
--
作者：
Hashimoto, Naoki;Kurita, Yoshihisa;Wada, Hiroshi
鳃盖是腹足类软体动物的一种新结构。由于鳃盖显示出与壳板的显著相似性，我们问这两个分泌器官之间是否存在进化联系。我们发现，一些参与壳场发展的基因在鳃盖中表达，如dpp和grainyhead，而engrailed和Hox 1没有。通过注射双链RNA（dsRNA）特异性敲低dpp导致壳板畸形。由于壳场边缘细胞增殖活化失败，壳板较小。dpp-dsRNA抑制了壳田边缘的grainyhead和chitin synthase 1的表达。然而，基质分泌并没有完全消除，铁蛋白，enrailed或Hox 1的表达不受dpp-dsRNA的影响，表明DPP是部分参与壳基质分泌的发育途径。我们还提出证据表明，dpp在鳃盖发育中起着关键作用。事实上，dpp-dsRNA损害了鳃盖中的基质分泌以及grainyhead的表达。基于这些观察，即DPP是重要的发展，双方的壳板和鳃盖，我们得出的结论是，共同选择的DPP的后部的脚有助于在腹足动物的鳃盖的创新。(C)2012 Elsevier Inc. All rights reserved.
The operculum is a novel structure in gastropod molluscs. Because the operculum shows notable similarities to the shell plate, we asked whether there were an evolutionary link between these two secretory organs. We found that some of the genes involved in shell-field development are expressed in the operculum, such as dpp and grainyhead, whereas engrailed and Hox1 are not. Specific knockdown of dpp by injection of double-stranded RNA (dsRNA) resulted in malformation of the shell plate. The shell plate was smaller due to failure of activation of cell proliferation in the shell-field margin. The expressions of grainyhead and chitin synthase 1 in the shell field margin were suppressed by dpp-dsRNA. However, matrix secretion was not completely abolished, and the expressions of ferritin, engrailed or Hox1 were not affected by dpp-dsRNA, indicating that dpp is partly involved in the developmental pathway for shell matrix secretion. We also present evidence that dpp performs a key role in operculum development. Indeed, dpp-dsRNA impaired matrix secretion in the operculum as well as expression of grainyhead. Based on these observations that dpp is important for development of both the shell plate and operculum, we conclude that co-option of dpp to the posterior part of the foot contributed to the innovation of the operculum in gastropods. (C) 2012 Elsevier Inc. All rights reserved.