Transforming growth factor-beta modulation of glycosaminoglycan production by mesenchymal cells of the developing murine secondary palate.
Transforming growth factor-beta modulation of glycosaminoglycan production by mesenchymal cells of the developing murine secondary palate.
复制标题
转化生长因子-β对发育中的小鼠次级腭的间充质细胞产生糖胺聚糖的调节。
DOI：
10.1016/0012-1606(91)90136-q
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发表时间：
1991
影响因子：
2.7
通讯作者：
Greene,RM
中科院分区：
文献类型：
--
作者：
D'Angelo,M;Greene,RM
哺乳动物次级腭部的发育需要适当的细胞外基质，特别是糖胺多聚糖(GAG)和胶原蛋白。调节这些分子新陈代谢的内源性因素在很大程度上是未知的。转化生长因子β-1(转化生长因子-β-1)是局部衍生分子的候选分子，因为它可以调节多种细胞系的细胞外基质代谢。因此，我们试图确定转化生长因子β1在调节小鼠胚胎腭部间充质细胞产生和降解GAG中的调节作用。用转化生长因子β-1或转化生长因子β-2处理，但不用IGFII处理，可刺激总GAG合成。此外，经转化生长因子β-1和转化生长因子α共同作用的细胞，经转化生长因子-β-1处理后，GAG合成呈协同增加，而转化生长因子-α则无此作用。转化生长因子β-1和转化生长因子-β-2同时刺激未引起协同反应。这些研究表明，由胚胎腭部细胞合成的转化生长因子β能够特异性地刺激MEPM细胞合成GAG。发育中的头面部存在的其他生长因子也可能调节转化生长因子β诱导的GAG合成，这是一个对胚胎腭部正常发育至关重要的生物合成过程。
Development of the mammalian secondary palate requires proper production of the extracellular matrix, particularly glycosaminoglycans (GAGs) and collagen. Endogenous factors that regulate the metabolism of these molecules are largely undefined. A candidate for a locally derived molecule would be transforming growth factorβ1(TGFβ1) by virtue of its potency as a modulator of extracellular matrix metabolism by several cell lines. We have thus attempted to assign a regulatory role for TGFβ1in modulation of GAG production and degradation by mesenchymal cells of the murine embryonic palate (MEPM). Treatment with TGFβ1or TGFβ2, but not IGF-II, resulted in a stimulation of total GAG synthesis. Furthermore, cells treated withbothTGFβ1and TGFα showed a synergistic increase in GAG synthesis if pretreated with TGFβ1but not TGFα. Simultaneous stimulation with TGFβ1and TGFβ2did not elicit a synergistic response. These studies demonstrate the ability of TGFβ, synthesized by embryonic palatal cells, to specifically stimulate GAG synthesis by MEPM cells. Other growth factors present in the developing craniofacial region may also modulate TGFβ-induced GAG synthesis, a biosynthetic process critical to normal development of the embryonic palate.