CHARACTERIZATION OF THE MOUSE CDNA AND GENE CODING FOR A HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN - EXPRESSION DURING DEVELOPMENT
CHARACTERIZATION OF THE MOUSE CDNA AND GENE CODING FOR A HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN - EXPRESSION DURING DEVELOPMENT
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DOI：
10.1021/bi00104a030
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发表时间：
1991-10-08
期刊：
影响因子：
2.9
通讯作者：
JAMISON, CS
中科院分区：
文献类型：
--
作者：
DEGEN, SJF;STUART, LA;JAMISON, CS
分离并鉴定了编码小鼠肝细胞生长因子样蛋白（HGF 样蛋白）的 cDNA 和基因。 从转录起始位点到多聚腺苷酸化位点的基因长度为 4613 bp，由 18 个外显子组成，并由 17 个插入序列分隔。 外显子的长度范围为 36 至 227 bp，而插入序列的长度范围为 78 至 613 bp。 使用从小鼠肝脏分离的总RNA通过引物延伸分析来鉴定转录起始位点。 根据这些结果，第一个外显子长度为 146 bp，包含 94 bp 5'-非编码序列。 序列 5'TATGTG3' 存在于转录起始位点上游 34 和 39 bp 之间，并且可能是在许多组成型表达的真核基因中发现的 TATA 序列，作为 RNA 聚合酶 II 的启动子。 -96至-92处的序列5'GCAAT3'可能是负责刺激某些真核基因转录的CCAAT序列。 Genbank 和 NBRF 数据库中的相同序列与编码人和小鼠 HGF 样蛋白的基因中的相似区域同源（Han 等，1991）。 酰基肽水解酶基因位于小鼠 HGF 样蛋白下游 410 bp，但从互补链转录。 HGF 样蛋白的小鼠 cDNA 编码的推定蛋白与其人类同源物具有相同的结构域结构，具有四个 kringle 结构域，后跟一个丝氨酸蛋白酶样结构域。 根据cDNA翻译序列，小鼠HGF样蛋白长度为716个氨基酸，分子量为80K。 有四个潜在的 N 连接碳水化合物附着位点。 将小鼠 HGF 样蛋白的 DNA 和氨基酸序列与人类蛋白进行比较。 总体而言，这两种蛋白质彼此大约 80% 相同。 人类 HGF 样蛋白的 mRNA 在人类肝脏中的长度为 2.4 和 3.0 kb，与之相反，在小鼠和大鼠的肝脏中只发现了较小的种类。 通过Northern分析确定发育期间和母鼠中编码HGF样蛋白的mRNA的表达模式。 显然，编码 HGF 样蛋白的大部分 mRNA 在肝脏中表达。 信使 RNA 在肺、肾上腺和胎盘中也以较低水平表达。
The cDNA and gene coding for mouse hepatocyte growth factor-like protein (HGF-like protein) were isolated and characterized. The size of the gene from the site of initiation of transcription to the polyadenylation site is 4613 bp in length and is composed of 18 exons separated by 17 intervening sequences. The exons range in size from 36 to 227 bp in length, while the intervening sequences range in size from 78 to 613 bp in length. The site of initiation of transcription was identified by primer extension analysis using total RNA isolated from mouse liver. On the basis of these results, the first exon is 146 bp in length and includes 94 bp of 5'-noncoding sequence. The sequence 5'TATGTG3' is present between 34 and 39 bp upstream of the transcription start site and could potentially be the TATA sequence found for many constitutively expressed eukaryotic genes to be the promoter for RNA polymerase II. The sequence 5'GCAAT3' at -96 to -92 may be the CCAAT sequence responsible for stimulation of transcription of some eukaryotic genes. The same sequences in the Genbank and NBRF databases were homologous to similar regions in the genes coding for both human and mouse HGF-like protein (Han et al., 1991). The acyl-peptide hydrolase gene is 410 bp downstream of the mouse HGF-like protein, but is transcribed from the complementary strand. The mouse cDNA for HGF-like protein codes for a putative protein with the same domain structure as its human homologue with four kringle domains followed by a serine protease-like domain. On the basis of the translated sequence of the cDNA, the mouse HGF-like protein would be 716 amino acids in length with a molecular weight of 80K. There are four potential N-linked carbohydrate attachment sites. The DNA and amino acid sequences of mouse HGF-like protein are compared to the human protein. Overall, the two proteins are about 80% identical with each other. In contrast to mRNA for human HGF-like protein, which is 2.4 and 3.0 kilobases in length in human liver, only the smaller species is seen in mouse and rat liver. The expression pattern of mRNA coding for HGF-like protein during development and in maternal rats was determined by Northern analysis. It is apparent that the majority of mRNA coding for HGF-like protein is expressed in liver. Messenger RNA is also expressed at a lower level in lung, adrenal, and placenta.