Photoprotection in the green tidal alga Ulva prolifera: role of LHCSR and PsbS proteins in response to high light stress.
Photoprotection in the green tidal alga Ulva prolifera: role of LHCSR and PsbS proteins in response to high light stress.
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DOI：
10.1111/j.1438-8677.2012.00712.x
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发表时间：
2013-11
期刊：
影响因子：
3.9
通讯作者：
S. Mou;X. Zhang;M. Dong;X. Fan;J. Xu;S. Cao;D. Xu;W. Wang;N. Ye
中科院分区：
文献类型：
--
作者：
S. Mou;X. Zhang;M. Dong;X. Fan;J. Xu;S. Cao;D. Xu;W. Wang;N. Ye
石莼，潮间带macrophages，必须适应广泛的变化，在光强度，使这个物种特别值得研究的光保护机制的演变。强光诱导增加非光化学猝灭（NPQ）和刺激叶黄素循环组分的脱环氧化，而DTT处理的样品具有较低的NPQ能力，表明叶黄素循环必须参与光保护。在这项工作中，我们发现，PsbS相关的NPQ保持在美国。增殖。基因表达分析表明，LhcSR和psbS基因在强光下表达上调，说明这两个基因是光诱导的。LHCSR和PsbS蛋白在不同光强下均有表达，且在强光条件下有积累，且PsbS含量高于LHCSR，说明U. prolifera更依赖于PsbS蛋白浓度。此外，LHCSR和PsbS蛋白的水平是高的，即使在黑暗中，无论是转录水平还是LhcSR和psbS基因的蛋白质含量变化显着短期强光照射后。这些研究结果表明，这种植物可以通过调节叶黄素循环和PsbS和/或LHCSR的浓度来调节NPQ水平。
Ulva prolifera, an intertidal macroalga, has to adapt to wide variations in light intensity, making this species particularly rewarding for studying the evolution of photoprotective mechanisms. Intense light induced increased non-photochemical quenching (NPQ) and stimulated de-epoxidation of xanthophyll cycle components, while DTT-treated samples had lower NPQ capacity, indicating that the xanthophyll cycle must participate in photoprotection. In this work, we found that the PsbS-related NPQ was maintained in U. prolifera. According to analysed gene expression, both LhcSR and psbS were up-regulated in high light, suggesting that these two genes are light-induced. LHCSR and PsbS proteins were present at different light intensities and accumulated under high light conditions, and PsbS concentrations were higher than LHCSR, showing that the NPQ mechanism of U. prolifera is more dependent on PsbS protein concentration. Moreover, the level of both LHCSR and PsbS proteins was high even in the darkness, and neither the transcript level nor protein content of LhcSR and psbS genes varied significantly following short-term exposure to intense light. These findings suggest that this alga can modulate NPQ levels through regulation of the xanthophyll cycle and concentrations of PsbS and/or LHCSR.