Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR
Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR
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DOI：
10.1128/jcm.40.3.863-867.2002
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发表时间：
2002-03-01
影响因子：
9.4
通讯作者：
Kudo, T
中科院分区：
文献类型：
--
作者：
Nagao, S;Yoshinouchi, M;Kudo, T
采用快速定量实时 PCR 方法对 E2 和 E6 基因的拷贝数进行定量，以分析人乳头瘤病毒 16 型 (HPV-16) DNA 的物理状态。当实验模型中超过 40% 的 HPV-161 DNA 与 E2 基因破坏整合时，发现两个拷贝数存在显着差异。 50 份临床样本中 HPV-16 DNA 的物理状态为 21 例 (42%) 完全游离型、14 例 (28%) 伴随型、15 例 (30%) 整合型。整合型和/或伴随型 HPV-16 DNA 的患病率随着宫颈疾病的进展而增加。 11 例宫颈上皮内瘤变中有 4 例部分或完全涉及 HPV-16 DNA 的整合形式。这种快速、灵敏的技术可用于分析 HPV DNA 的物理状态。
A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV- 161 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.