Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus.
Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus.
复制标题
DOI：
10.1186/1743-422x-8-412
复制
发表时间：
2011-08-21
期刊：
影响因子：
4.8
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Hunter DA;Fletcher JD;Davies KM;Zhang H
水仙花是世界上最受欢迎的观赏植物之一。它们还为研究负责黄色和橙子花色的类胡萝卜素色素提供了科学模型。在反双色水仙花中，黄色的花喇叭随着年龄的增长而褪色为白色。这种类型的水仙花特别容易变色，因此，在开放时，可以观察到花被的黄色被“打破”成白色的斑块。这种变色症状是其他植物如郁金香中马铃薯Y病毒感染的特征，郁金香的变色是由于花青素的存在改变。然而，反向双色花显示颜色断裂没有显示其他病毒样症状，如叶斑驳或植物发育迟缓，导致一些人认为反向双色花中基于类胡萝卜素的颜色断裂可能不是由病毒感染引起的。虽然马铃薯Y病毒已被报道会导致其他花卉品种的颜色断裂，酶联免疫测定与特异性的马铃薯Y病毒科的抗体表明，马铃薯Y病毒不负责发生颜色断裂在反向双色水仙花。这种类型的水仙花的颜色断裂显然与花被片中存在大量长度为502-580 nm的杆状病毒颗粒有关。从花汁液显示颜色断裂引起红色坏死病变的Gomphrena globosa，表明马铃薯X病毒的存在。当使用不显示颜色断裂的反双色花的汁液时，在该指示植物中未观察到红色坏死病变。逆转录酶聚合酶反应，使用简并引物卡拉，potex和马铃薯Y病毒连接的病毒RNA与颜色断裂和测序的扩增产物表明，马铃薯X病毒水仙花叶病毒是主要的病毒与颜色断裂的发生。高病毒计数与反向双色水仙花有关，这些花显示颜色断裂，但没有显示其他感染症状。水仙花叶病毒是一种与类胡萝卜素颜色突变明显相关的病毒。
Daffodils (Narcissus pseudonarcissus) are one of the world's most popular ornamentals. They also provide a scientific model for studying the carotenoid pigments responsible for their yellow and orange flower colours. In reverse bicolour daffodils, the yellow flower trumpet fades to white with age. The flowers of this type of daffodil are particularly prone to colour break whereby, upon opening, the yellow colour of the perianth is observed to be 'broken' into patches of white. This colour break symptom is characteristic of potyviral infections in other ornamentals such as tulips whose colour break is due to alterations in the presence of anthocyanins. However, reverse bicolour flowers displaying colour break show no other virus-like symptoms such as leaf mottling or plant stunting, leading some to argue that the carotenoid-based colour breaking in reverse bicolour flowers may not be caused by virus infection. Although potyviruses have been reported to cause colour break in other flower species, enzyme-linked-immunoassays with an antibody specific to the potyviral family showed that potyviruses were not responsible for the occurrence of colour break in reverse bicolour daffodils. Colour break in this type of daffodil was clearly associated with the presence of large quantities of rod-shaped viral particles of lengths 502-580 nm in tepals. Sap from flowers displaying colour break caused red necrotic lesions on Gomphrena globosa, suggesting the presence of potexvirus. Red necrotic lesions were not observed in this indicator plant when sap from reverse bicolour flowers not showing colour break was used. The reverse transcriptase polymerase reactions using degenerate primers to carla-, potex- and poty-viruses linked viral RNA with colour break and sequencing of the amplified products indicated that the potexvirus Narcissisus mosaic virus was the predominant virus associated with the occurrence of the colour break. High viral counts were associated with the reverse bicolour daffodil flowers that were displaying colour break but otherwise showed no other symptoms of infection. Narcissus mosaic virus was the virus that was clearly linked to the carotenoid-based colour break.