Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae.
Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae.
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DOI：
10.1111/nph.16392
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发表时间：
2020-04
期刊：
影响因子：
--
通讯作者：
Lepetit M
中科院分区：
文献类型：
--
作者：
Boivin S;Ait Lahmidi N;Sherlock D;Bonhomme M;Dijon D;Heulin-Gotty K;Le-Queré A;Pervent M;Tauzin M;Carlsson G;Jensen E;Journet EP;Lopez-Bellido R;Seidenglanz M;Marinkovic J;Colella S;Brunel B;Young P;Lepetit M
豆科豆科植物如豌豆和蚕豆等形成共生根瘤，土壤中含有大量的豆科共生根瘤菌(Rhizobium legumosarum symbiovar viciae，RLV)。然而，竞争形成根瘤(CFN)的细菌通常不是最有效地固定氮素的细菌，导致豆类作物产量下降。在这里，我们研究了寄主植物对RLV多样性的差异选择。通过用豌豆和蚕豆从欧洲五个地点的土壤中捕获根瘤，收集了大量的RLV。对具有代表性的基因组进行了测序。同时，利用MetabarCoding直接估计了这些土壤中RLV的多样性和丰度。测定了两种豆科植物寄主的CFN。豌豆/蚕豆CFN与RLV基因组区域相关。豌豆和/或蚕豆根瘤中细菌的CFN变异解释了豌豆和蚕豆根瘤中RLV基因的不同丰度。没有发现CFN与核心基因组变异之间存在遗传关联的证据，但NOD基因特定区域的变异以及其他质粒基因座的变异与CFN的差异有关。这些发现揭示了RLV中CFN的遗传控制，并强调了寄主植物在控制根瘤菌多样性方面的重要性。
Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity.
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影响因子：
3.6
作者：
FIRMIN, JL;WILSON, KE;DOWNIE, JA
通讯作者：
DOWNIE, JA
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AMARGER, N
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AMARGER, N
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4.4
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Amarger, N
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作者：
ECONOMOU, A;HAMILTON, WDO;DOWNIE, JA
通讯作者：
DOWNIE, JA