Micro-vesicles derived from bone marrow stem cells protect the kidney both in vivo and in vitro by microRNA-dependent repairing
Micro-vesicles derived from bone marrow stem cells protect the kidney both in vivo and in vitro by microRNA-dependent repairing
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源自骨髓干细胞的微囊泡通过 microRNA 依赖性修复在体内和体外保护肾脏。
DOI：
10.1111/nep.12490
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发表时间：
2015-09-01
期刊：
影响因子：
2.5
通讯作者：
Zhao, Weihong
中科院分区：
文献类型：
--
作者：
He, Juan;Wang, Yan;Zhao, Weihong
来自骨间充质干细胞（MSCs）的微囊泡（MVs）已被证明有助于损伤肾脏的恢复。本研究的目的是探讨MVs的生物学效应和修复机制。方法从MSC上清液中获得微囊泡。在体外，用转化生长因子（TGF-1）处理近端小管上皮细胞（HK-2）。检测E-cadherin和-平滑肌肌动蛋白（-SMA）的表达。体外将小鼠分为：对照组、单侧输尿管梗阻（UUO）组、UUO+MSC组和UUO+MV组。术后注射mv和MSCs。小鼠在手术后7/14天被杀死，并进行进一步的检查。使用miRCURY Hy3/Hy5 Power标记试剂盒标记微rna表达，并在miRCURY LNA Array上进行杂交。结果在体外，MV可逆转TGF-1诱导的HK2细胞形态变化，稳定E-cadherin的表达，减少-SMA的分泌。在体内，MV和MSC组的血尿素氮（BUN）水平低于UUO组(P
AimsMicro-vesicles (MVs) from bone mesenchymal stem cells (MSCs) have been shown to contribute to the recovery of damaged kidney. The aims of the present study are to investigate the biological effects and repair mechanisms of MVs.MethodsMicro-vesicles were obtained from MSC supernatants. In vitro, the proximal tubular epithelial cells (HK-2) were treated with transforming growth factor (TGF-1). The expressions of E-cadherin and -smooth muscle actin (-SMA) were evaluated. In vitro, the mice were divided into: control, unilateral ureteral obstruction (UUO), UUO+MSC, and UUO+MV group. MVs and MSCs were injected after surgery. The mice were killed 7/14 days after surgery and handled for further tests. The micro-RNA expressions were labeled using the miRCURY Hy3/Hy5 Power labeling kit and hybridized on the miRCURY LNA Array.ResultsIn vitro, MV reversed transforming growth factor-1 (TGF-1)-induced morphological changes, and firmed the expression of E-cadherin and reduced the secretion of -SMA in HK2 cells. In vivo, the level of blood urea nitrogen (BUN) in the MV and MSC group was lower than the UUO (P