THE YEAST SCG1 GENE - A G-ALPHA-LIKE PROTEIN IMPLICATED IN THE A-FACTOR AND ALPHA-FACTOR RESPONSE PATHWAY
THE YEAST SCG1 GENE - A G-ALPHA-LIKE PROTEIN IMPLICATED IN THE A-FACTOR AND ALPHA-FACTOR RESPONSE PATHWAY
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DOI：
10.1016/0092-8674(87)90166-8
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发表时间：
1987-09-25
期刊：
影响因子：
64.5
通讯作者：
KURJAN, J
中科院分区：
文献类型：
--
作者：
DIETZEL, C;KURJAN, J
我们已经确定了SCG 1基因的能力，抑制信息素超敏感的sst 2 -1突变。SCG 1的核苷酸序列表明，它编码与α.脊椎动物G蛋白转导素的亚基、Gs、Gi和Go。SCG 1表达和功能是单倍体特异性的;单倍体scg 1细胞生长成由大的、异常形状的细胞组成的非常小的集落，而a/αscg 1/scg 1二倍体显示野生型形态、生长和孢子形成。 我们假设，SCG 1产品参与信息素反应途径，并提出了两个模型的SCG 1产品的功能。大鼠α s基因在酵母中的表达部分地补充了sst 2和scg 1缺陷，表明SCG 1和哺乳动物G α s基因之间高度保守的序列和功能。亚单位。
We have identified the SCG1 gene by its ability to suppress the pheromone-supersensitive sst2-1 mutation. The nucleotide sequence of SCG1 suggests that it encodes a 54 kd protein homologous to the .alpha. subunit of the vertebrate G proteins transducin, Gs, Gi, and Go. SCG1 expression and function are haploid-specific; haploid scg1 cells grow into very small colonies consisting of large, abnormally shaped cells, whereas a/.alpha. scg1/scg1 diploids show wild-type morphology, growth, and sporulation. We postulate that the SCG1 product is involved in the pheromone response pathway, and propose two models for the function of the SCG1 product. Expression of the rat .alpha.s gene in yeast partially complements both the sst2 and scg1 defects, indicating a high level of conservation of sequence and function between SCG1 and mammalian G.alpha. subunits.