Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves' disease.
Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves' disease.
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DOI：
10.1016/j.bbrc.2004.06.162
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发表时间：
2004-08
影响因子：
3.1
通讯作者：
T. Ohkura;Shin‐ichi Taniguchi;Kazuhiro Yamada;N. Nishio;T. Okamura;Akio Yoshida;Keiichi Kamijou;S. Fukata;K. Kuma;Y. Inoue;I. Hisatome;S. Senju;Y. Nishimura;C. Shigemasa
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文献类型：
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作者：
T. Ohkura;Shin‐ichi Taniguchi;Kazuhiro Yamada;N. Nishio;T. Okamura;Akio Yoshida;Keiichi Kamijou;S. Fukata;K. Kuma;Y. Inoue;I. Hisatome;S. Senju;Y. Nishimura;C. Shigemasa
具有卷曲螺旋结构域和锚蛋白重复序列的葡萄膜自身抗原（UACA）是患有全葡萄膜炎如Vogt-Koyanagi-Harada病的患者中的自身抗原。葡萄膜炎患者中IgG抗UACA抗体的流行率显著高于健康对照，表明其作为自身抗原的潜在作用。最初，UACA是在TSH刺激后从狗的甲状腺组织中克隆的。因此，我们推测UACA可能是自身免疫性甲状腺疾病的一种新的自身抗原。我们用ELISA法测定了自身免疫性甲状腺疾病（Graves病、桥本甲状腺炎、亚急性甲状腺炎和无症状甲状腺炎）患者的血清抗UACA抗体滴度。Graves病组抗UACA抗体阳性率为15%，明显高于健康对照组（0%）。Graves眼病患者抗UACA抗体阳性率明显高于无眼病的Graves病患者（29%比11%）。特别是75%的重症眼肌病患者UACA滴度增高。免疫组织化学分析显示，UACA蛋白表达在眼肌以及人甲状腺滤泡细胞。总之，UACA是甲状腺相关眼病眼肌自身抗原的新候选者。
Uveal autoantigen with coiled coil domains and ankyrin repeats (UACA) is an autoantigen in patients with panuveitis such as Vogt–Koyanagi–Harada disease. The prevalence of IgG anti-UACA antibodies in patients with uveitis is significantly higher than healthy controls, suggesting its potential role as an autoantigen. Originally, UACA was cloned from dog thyroid tissue following TSH stimulation. So, we presumed UACA could be a novel autoantigen in autoimmune thyroid diseases. We measured serum anti-UACA antibody titer using ELISA in patients with autoimmune thyroid diseases (Graves’ disease, Hashimoto’s thyroiditis, subacute thyroiditis, and silent thyroiditis). The prevalence of anti-UACA antibodies in Graves’ disease group was significantly higher than that in healthy group (15% vs. 0%). Moreover, the prevalence of anti-UACA antibodies in Graves’ ophthalmopathy was significantly higher than that in Graves’ patients without ophthalmopathy (29% vs. 11%). Especially, 75% of severe ocular myopathy cases showed high UACA titer. Immunohistochemical analysis revealed that UACA protein is expressed in eye muscles as well as human thyroid follicular cells. Taken together, UACA is a novel candidate for eye muscle autoantigens in thyroid-associated ophthalmopathy.