Circulating methylated DNA
Circulating methylated DNA
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DOI：
10.1196/annals.1318.006
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Herman, JG
中科院分区：
文献类型：
--
作者：
Herman, JG
癌症的早期发现将有助于更好地管理患者，因为生存率受疾病分期的影响很大。循环核酸代表了可能用于癌症早期检测的生物标志物。此外，这些生物标志物可能用于跟踪癌症患者的进展。基因的启动子区域CpG岛的DNA甲基化代表了一种这样的基于核酸的标记，并且具有优于其他方法的一些优点。这些包括DNA作为分子的稳定性，使用简单的基于聚合酶链反应的方法检测这种变化的能力，以及甲基化DNA优先存在于血浆或血清中的核小体中的潜力。这些因素，并证明其对血液中循环的甲基化DNA的检测的影响。
Early detection of cancer would facilitate better management of patients, because survival is greatly affected by stage of presentation. Circulating nucleic acids represent a biomarker that might be used in the early detection of cancer. In addition, such biomarkers could potentially be used to follow the progression of patients with cancer. DNA methylation of the promoter region CpG islands of genes represents one such nucleic acid-based marker, and possesses some advantages over other approaches. These include the stability of DNA as a molecule, the ability to detect such changes using simple polymerase chain reaction-based approaches, and the potential for methylated DNA to be preferentially present in nucleosomes in plasma or serum. These factors are presented and their impact on the detection of methylated DNA circulating in the bloodstream is demonstrated.