Neurokinin-1 receptor and substance P messenger RNA levels increase during intraabdominal adhesion formation
Neurokinin-1 receptor and substance P messenger RNA levels increase during intraabdominal adhesion formation
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DOI：
10.1006/jsre.2002.6533
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Becker, JM
中科院分区：
文献类型：
--
作者：
Reed, KL;Fruin, AB;Becker, JM
背景。腹膜内粘连是术后并发症的重要原因，也是腹部手术的主要长期并发症之一。在分子水平上，粘附的形成涉及细胞因子、生长因子、细胞粘附分子和神经肽以及靠近创伤区域的细胞分泌的许多其他因素的复杂相互作用。目前研究粘附形成过程的分子过程的研究有限。因此，本研究的目的是确定大鼠黏附形成早期P物质、神经激肽-1受体和P物质作用的下游介质的基因表达模式。在雄性Wistar大鼠腹膜一侧形成4个缺血钮扣。分别于术后3、6、12小时和1、3、5、7天处死动物。从缺血钮扣和相对侧壁的腹膜组织中采集总RNA分离。采用半定量RT-PCR检测神经激肽-1受体、P物质、tgf -1、细胞粘附分子ICAM-1和vcam -1在转录物水平上的变化。与对照组相比，缺血钮扣部位腹膜组织中P物质、神经激肽-1受体、tgf β -1、ICAM-1和VCAM-1的信使RNA水平显著升高（P < 0.05）。在对侧壁腹膜组织中，早期P物质mRNA水平显著升高（P < 0.05）。tgf - β 1、神经激肽-1受体和ICAM-1 mRNA水平与对照组相比显著升高（P < 0.05），而VCAM-1 mRNA水平无显著变化。腹膜组织中与腹腔粘连相关的P物质、神经激肽-1受体以及P物质作用的下游介质TGF-β1、ICAM-1和VCAM-1 mRNA水平升高，支持P物质在粘连形成中的作用。(C) 2002 Elsevier Science （USA）。
Background. Intraabdominal peritoneal adhesions are a significant cause of postoperative morbidity and remain one of the major long-term complications associated with abdominal surgery. Adhesion formation at the molecular level involves a complex interaction of cytokines, growth factors, cell adhesion molecules, and neuropeptides, as well as many other factors secreted by cells proximate to the traumatized area. Limited studies exist which investigate the molecular processes involved in adhesion formation. Therefore, the aim of the present study was to determine the pattern of gene expression for substance P, the neurokinin-1 receptor, and downstream mediators of substance P action during the early stages of adhesion formation in the rat.Methods. Four ischemic buttons were created on one side of the peritoneum in male Wistar rats. Animals were sacrificed at 3, 6, and 12 h and 1, 3, 5, and 7 days following surgery. Peritoneal tissue from ischemic buttons and from the opposite sidewall was harvested for total RNA isolation. Semiquantitative RT-PCR was used to measure changes at the transcript level for the neurokinin-1 receptor, substance P, TGFbeta-1, and the cell adhesion molecules ICAM-1 and VCAM-1.Results. Messenger RNA levels for substance P, neurokinin-1 receptor, TGFbeta-1, ICAM-1, and VCAM-1 were significantly increased in peritoneal tissue taken from ischemic button sites (P < 0.05) when compared with controls. In the peritoneal tissues taken from the opposite sidewall, there was a significant (P < 0.05) early increase in substance P mRNA levels. TGF-beta1, neurokinin-1 receptor, and ICAM-1 mRNA levels were also significantly (P < 0.05) increased when compared to controls, while the mRNA levels for VCAM-1 did not change.Conclusions. The increased levels of mRNA for substance P, the neurokinin-1 receptor, and the downstream mediators of substance P action, TGF-β1, ICAM-1, and VCAM-1, in peritoneal tissue associated with intraabdominal adhesions support a role for substance P in adhesion formation. (C) 2002 Elsevier Science (USA).