Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies.
Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies.
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通过微雕刻同时检测人类淋巴细胞的分泌产物：细胞因子和抗原反应性抗体。
DOI：
10.1016/j.clim.2008.06.009
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Love JC
中科院分区：
文献类型：
--
作者：
Bradshaw EM;Kent SC;Tripuraneni V;Orban T;Ploegh HL;Hafler DA;Love JC
造血细胞分泌的细胞表面决定因素、细胞因子和抗体被用来对其谱系和功能进行分类。目前可用的技术无法阐明多种分泌蛋白，同时也无法将表型表面显示标记分配给单个活细胞。在这里，一种软光刻方法，微雕刻，适用于对单个人外周血单核细胞的分泌细胞因子（IFN-γ和IL-6），抗原特异性抗体和谱系特异性表面表达标记的多重询问。将该方法应用于抗胰岛素抗体阳性的新发1型糖尿病患者的临床样本显示，约0.58%的循环CD19+ B细胞分泌IgG同型的胰岛素反应原抗体，2-3%的循环细胞分泌IL-6。这些数据证明了微雕刻在同时询问单个人类细胞的多种表型以及通过其分泌产物检测罕见细胞群方面的效用。
Cell surface determinants, cytokines and antibodies secreted by hematopoietic cells are used to classify their lineage and function. Currently available techniques are unable to elucidate multiple secreted proteins while also assigning phenotypic surface-displayed markers to the individual living cells. Here, a soft lithographic method, microengraving, was adapted for the multiplexed interrogation of populations of individual human peripheral blood mononuclear cells for secreted cytokines (IFN-γ and IL-6), antigen-specific antibodies, and lineage-specific surface-expressed markers. Application of the method to a clinical sample from a recent onset Type 1 diabetic subject with a positive titer of anti-insulin antibodies showed that ~0.58% of circulating CD19+ B cells secreted proinsulin-reactive antibodies of the IgG isotype and 2–3% of circulating cells secreted IL-6. These data demonstrate the utility of microengraving for interrogating multiple phenotypes of single human cells concurrently and for detecting rare populations of cells by their secreted products.
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影响因子：
30.5
作者：
Acosta-Rodriguez, Eva V.;Napolitani, Giorgio;Sallusto, Federica
通讯作者：
Sallusto, Federica
影响因子：
46.9
作者：
Love, J. Christopher;Ronan, Jehnna L.;Ploegh, Hidde L.
通讯作者：
Ploegh, Hidde L.
DOI：
10.1073/pnas.0502323102
发表时间：
2005-05-17
影响因子：
11.1
作者：
Burgoon, MP;Keays, KM;Gilden, DH
通讯作者：
Gilden, DH
影响因子：
5.4
作者：
Chen, ZJ;Shimizu, F;Notkins, AL
通讯作者：
Notkins, AL
影响因子：
2.2
作者：
Townsend, SE;Goodnow, CC;Cornall, RJ
通讯作者：
Cornall, RJ