Stability of serum, plasma and urine osmolality in different storage conditions: Relevance of temperature and centrifugation
Stability of serum, plasma and urine osmolality in different storage conditions: Relevance of temperature and centrifugation
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DOI：
10.1016/j.clinbiochem.2017.03.019
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发表时间：
2017-09-01
影响因子：
2.8
通讯作者：
Miguel Bauca, Josep
中科院分区：
文献类型：
--
作者：
Sureda-Vives, Macia;Morell-Garcia, Daniel;Miguel Bauca, Josep
Background: Osmolality reflects the concentration of all dissolved particles in a body fluid, and its measurement is routinely performed in clinical laboratories for the differential diagnosis of disorders related with the hydrolytic balance regulation, the renal function and in small-molecule poisonings. The aim of the study was to assess the stability of serum, plasma and urine osmolality through time and under different common storage conditions, including delayed centrifugation.Methods: Blood and urine samples were collected, and classified into different groups according to several preanalytical variables: serum or plasma lithium-heparin tubes; spun or unspun; stored at room temperature (RT), at 4 degrees C or frozen at - 21 degrees C. Aliquots from each group were assayed over time, for up to 14 days. Statistical differences were based on three different international performance criteria.Results: Whole blood stability was higher in the presence of anticoagulant. Serum osmolality was stable for 2 days at RT and 8 days at 4 degrees C, while plasma was less stable when refrigerated. Urine stability was 5 days at RT, 4 days at 4 degrees C and > 14 days when frozen.Discussion: Osmolality may be of great interest for the management of several conditions, such as in case of a delay in the clinical suspicion, or in case of problems in sample collection or processing. The ability to obtain reliable results for samples kept up to 14 days also offers the possibility to retrospectively assess baseline values for patients which may require it.
