Deproteinized bone with VEGF gene transfer to facilitate the repair of early avascular necrosis of femoral head of rabbit
Deproteinized bone with VEGF gene transfer to facilitate the repair of early avascular necrosis of femoral head of rabbit
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DOI：
10.3760/cma.j.issn.1008-1275.2009.05.003
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发表时间：
2009-10-01
影响因子：
2.1
通讯作者：
Han Zhi-min
中科院分区：
文献类型：
--
作者：
Cao Kai;Huang Wei;Han Zhi-min
目的：目的：探索一种治疗早期股骨头缺血性坏死（AVNFH）的新方法。A组将重组质粒pcDNA3.1-hVEGF 165吸附的脱蛋白骨（DPB）植入坏死股骨头钻孔隧道内。B组仅植入DPB。C组只钻取隧道，不植入DPB或质粒。分别于术后1、2、4、8、16周取股骨头标本。免疫组化法检测VEGF 165和I型胶原的表达。X线检查显示骨形成。结果：A组植入后2周可检测到VEGF 165的表达，其他各组均未见表达。术后2、4、8周A组Ⅰ型胶原表达与其他各组比较差异有显著性（P
Objective: To explore a new method for early avascular necrosis of femoral head (AVNFH) therapy.Methods: Sixty-nine AVNFH New Zealand adult rabbits were randomly divided into three groups with equal number. In Group A, deproteinized bone (DPB) that absorbed with recombinant plasmid pcDNA3.1-hVEGF165 was implanted into the drilled tunnel of necrotic femoral head. In Group B, only DPB was implanted. In Group C, only tunnel was drilled without DPB or plasmid implanted. Femoral head specimens were obtained at postoperative 1, 2, 4, 8, 16 weeks. The expression of VEGF165 and collagen I was detected by immunohistochemistry. Bone formation was detected generally by X-ray. Angiogenesis and the repair of the femoral head were observed histologically.Results: The expression of VEGF 165 could be detected 2 weeks after implantation in Group A, but it was not observed in other groups. The result of collagen I expression had a significantly difference 2, 4 and 8 weeks after operation in GroupA from those in other groups (P