Hox gene function and interaction in the milkweed bug Oncopeltus fasciatus (Hemiptera)
Hox gene function and interaction in the milkweed bug Oncopeltus fasciatus (Hemiptera)
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DOI：
10.1016/j.ydbio.2005.08.010
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发表时间：
2005-11-15
影响因子：
2.7
通讯作者：
Kaufman, TC
中科院分区：
文献类型：
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作者：
Angelini, DR;Liu, PZ;Kaufman, TC
对遗传模式生物（如果蝇）的研究表明，同源复合体（Hox）基因在胚胎发生过程中赋予片段同一性。对其他昆虫分类群的比较研究表明，Hox基因在沿前-后体轴的大部分保守区域表达，但它们是否在不同昆虫中发挥相同的功能是一个悬而未决的问题。大多数Hox基因仅在少数经历变态的全变态昆虫中进行了功能研究。因此，目前尚不清楚Hox基因在大多数直接发育的昆虫和其他节肢动物中是如何起作用的。为了解决这个问题，我们使用RNAi和原位杂交相结合的方法来揭示Hox基因在乳草虫Oncopeltus fasciatus中的表达、功能和调控相互作用。与果蝇相比，我们的研究结果揭示了许多相似之处和一些有趣的差异。我们发现基因Antennapedia是识别所有三个胸段所必需的，而Ultrabithorax、abinal - a和abinal - b共同塑造腹部。这三种腹部基因表现出与果蝇相似的后验流行，但显然是通过一些转录后机制。前文中已经揭示了头部基因proboscipedia、Deformed和Sex combs的功能，本研究发现pb在阴唇的复杂时间表达与其他昆虫相似，但其与Scr的调控关系是独特的。总的来说，我们的数据揭示了昆虫Hox基因的进化包括在表达和功能的一般保守中许多小的变化，并且马利筋虫为理解Hox基因在直接发育的昆虫中的作用提供了一个有用的模型。(C) 2005爱思唯尔公司版权所有。
Studies in genetic model organisms such as Drosophila have demonstrated that the homeotic complex (Hox) genes impart segmental identity during embryogenesis. Comparative studies in a wide range of other insect taxa have shown that the Hox genes are expressed in largely conserved domains along the anterior - posterior body axis, but whether they are performing the same functions in different insects is an open question. Most of the Hox genes have been studied functionally in only a few holometabolous insects that undergo metamorphosis. Thus, it is unclear how the Hox genes are functioning in the majority of direct developing insects and other arthropods. To address this question, we used a combination of RNAi and in situ hybridization to reveal the expression, functions, and regulatory interactions of the Hox genes in the milkweed bug Oncopeltus fasciatus. Our results reveal many similarities and some interesting differences compared to Drosophila. We find that the gene Antennapedia is required for the identity of all three thoracic segments, while Ultrabithorax, abdominal-A and Abdominal-B cooperate to pattern the abdomen. The three abdominal genes exhibit posterior prevalence like in Drosophila, but apparently via some post-transcriptional mechanism. The functions of the head genes proboscipedia, Deformed, and Sex combs reduced were shown previously, and here we find that the complex temporal expression of pb in the labium is like that of other insects, but its regulatory relationship with Scr is unique. Overall, our data reveal that the evolution of insect Hox genes has included many small changes within general conservation of expression and function, and that the milkweed bug provides a useful model for understanding the roles of Hox genes in a direct-developing insect. (C) 2005 Elsevier Inc. All rights reserved.