Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection
Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection
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DOI：
10.2116/analsci.25.1007
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发表时间：
2009-08-01
影响因子：
1.6
通讯作者：
Kusu, Fumiyo
中科院分区：
文献类型：
--
作者：
Kotani, Akira;Miyaguchi, Yuji;Kusu, Fumiyo
使用电化学检测高效液相色谱 (HPLC-ECD) 开发了一种测定大鼠和人类粪便中短链脂肪酸 (SCFA) 的简单方法。使用离子排斥柱和带有玻碳工作电极的电化学检测器制造了双通道 HPLC-ECD 系统。 0.1 mM HClO(4) 水溶液和含有2-甲基-1,4-萘醌的乙醇水溶液分别用作流动相和醌溶液。相对于 Ag/AgCl 电极，在 -0.9 V 检测电位下，乳酸、乙酸、丙酸、丁酸、异戊酸和戊酸的峰面积在 0.1 至 40 nmol 范围内与酸量呈线性关系。 4 nmol 的标准酸测定十次，相对标准偏差 (RSD) 小于 2.0%。健康人类粪便的分析结果可在 35 分钟内测量出来。所有 SCFA 的 RSD (n = 5) 均小于 2.7%，SCFA 的回收率超过 92%。该方法具有重现性好、操作简单、快速、无需分析物衍生等特点，具有临床和生物医学应用的潜力。
A simple method for determining short-chain fatty acids (SCFAs) in rat and human feces was developed using high-performance liquid chromatography with electrochemical detection (HPLC-ECD). A two-channel HPLC-ECD system was fabricated using an ion exclusion column and an electrochemical detector with a glassy carbon working electrode. Aqueous solutions of 0.1 mM HClO(4) and of ethanol containing 2-methyl-1,4-naphthoquinone served as a mobile phase and a quinone solution, respectively. Peak areas for lactic, acetic, propionic, butyric, isovaleric, and valeric acids at a detection potential of -0.9 V vs. an Ag/AgCl electrode showed a linear relationship with the acid amount in the range 0.1 to 40 nmol. Standard acids at 4 nmol were determined ten times with relative standard deviations (RSD) of less than 2.0%. The analytical results of healthy human feces were measured within 35 min. RSD (n = 5) in all SCFAs were less than 2.7%, and recoveries of SCFAs were more than 92%. The present method was characterized by reproducibility with the simple and rapid procedure without derivatization of analytes, and it has the potential for clinical and biomedical applications.