SUPRAMOLECULAR ORDERING OF DNA IN THE CHOLESTERIC LIQUID-CRYSTALLINE PHASE - AN ULTRASTRUCTURAL-STUDY
SUPRAMOLECULAR ORDERING OF DNA IN THE CHOLESTERIC LIQUID-CRYSTALLINE PHASE - AN ULTRASTRUCTURAL-STUDY
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DOI：
10.1016/s0006-3495(93)81063-4
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发表时间：
1993-07-01
影响因子：
3.4
通讯作者：
LIVOLANT, F
中科院分区：
文献类型：
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作者：
LEFORESTIER, A;LIVOLANT, F
146个碱基对DNA片段的水溶液在约160-290 mg/ml范围内形成胆甾酯液晶相。我们通过比较从偏光显微镜和电子显微镜获得的数据，对这一相进行了结构分析。这个阶段展示了呈现和解释的多个方面或“纹理”。它们主要取决于相对于观察面结构的取向，以及相中存在缺陷的性质、分布和数量。然后用这两种方法对这些缺陷进行分析，可以精确地确定其核心的分子取向。这种结构分析的生物学意义与对染色质结构和功能的理解有关。
Aqueous solutions of 146-base pair DNA fragments form a cholesteric liquid crystalline phase in the range of about 160-290 mg/ml. We present a structural analysis of this phase by comparing the data obtained from polarizing and electron microscopy. This phase shows multiple aspects or ''textures'' which are presented and interpreted. They mainly depend on the orientation of the structure relative to the observation plane and on the nature, distribution, and amount of defects present in the phase. These defects are then analyzed with the two methods, and the molecular orientations can be defined precisely in their core. The biological interest of such structural analyses is discussed in relation with the understanding of chromatin structure and function.