IMMUNOCYTOCHEMICAL LOCALIZATION OF ANGIOTENSINOGEN IN THE RAT-BRAIN
IMMUNOCYTOCHEMICAL LOCALIZATION OF ANGIOTENSINOGEN IN THE RAT-BRAIN
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DOI：
10.1016/0306-4522(88)90029-2
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发表时间：
1988-04-01
期刊：
影响因子：
3.3
通讯作者：
SERNIA, C
中科院分区：
文献类型：
--
作者：
THOMAS, WG;SERNIA, C
采用大鼠血浆血管紧张素原特异性抗血清，结合链霉素亲和素-生物素过氧化物酶敏感法，研究了大鼠脑内血管紧张素样免疫反应性的分布。放射免疫测定和Western blotting显示，血管紧张素原抗血清能识别大鼠血浆和脑脊液中的血管紧张素原，并与去ai血管紧张素原（100%）交叉反应，但与血管紧张素I和II、四肽、黄体生成素释放激素、大鼠白蛋白和其他8种血管紧张素原不发生交叉反应。血管紧张素样免疫反应在整个大鼠脑内的神经胶质细胞和神经元中均被检测到。神经胶质血管紧张素样免疫反应性在下丘脑和视前区浓度最高，中脑和髓脑浓度中等至高浓度。小脑颗粒层和纤维束可见神经胶质染色。大脑皮层和嗅球中很少见神经胶质染色。在整个苍白球和尾状壳核、丘脑的各个部位和下丘脑的视上核中观察到神经元免疫染色。中脑导水管周围中央灰质、中脑深部核、下丘及中脑前部散在细胞中可见中度免疫染色。在髓质，神经元染色定位于前庭核和主要在背外侧区域的其他细胞体。在小脑，染色主要见于小脑深部核和浦肯野细胞。大脑皮层的免疫染色局限于扣带皮层和初级嗅觉皮层。虹膜内皮层和外包膜附近散在的神经元呈浅染色。嗅球浅色神经元染色主要与二尖瓣细胞层有关。血管紧张素样免疫反应性的广泛分布支持了它在中枢神经系统合成并形成脑肾素-血管紧张素系统的一部分的观点。此外，它出现在正常情况下与血压、体液和电解质稳态控制相关的位点之外，这表明它的参与可能不限于这些调节功能。
The distribution of angiotensinogen-like immunoreactivity in the rat brain was investigated using specific antisera against pure rat plasma angiotensinogen in conjunction with the sensitive streptavidin-biotin peroxidase method. Angiotensinogen antisera were shown by radioimmunoassay and Western blotting to recognize angiotensinogen from both rat plasma and cerebrospinal fluid, and to cross-react with des-AI-angiotensinogen (100%) but not with angiotensin I and II, tetradecapeptide, luteinizing hormone-releasing hormone, rat albumin and angiotensinogen frm eight other species. Angiotensinogen-like immunoreactivity was detected throughout the rat brain in both neuroglia and neurons. The highest concentration of neuroglial angiotensinogen-like immunoreactivity was in the hypothalamus and preoptic areas, with moderate to heavy concentrations in the mesencephalon and myelencephalon. The cerebellum demonstrated neuroglial staining in the granular layer and fibre tracts. Very little neuroglial staining was noted in the cerebral cortex or olfactory bulbs. Neuronal immunostaining was observed throughout the globus pallidus and the caudate putamen, in various parts of the thalamus and the supraoptic nucleus of the hypothalamus. In the midbrain moderate immunostaining was observed in periaquaductal central gray, the deep mesencephalic nucleus, the inferior colliculus and in scattered cells in the anterior mesencephalon. In the medulla, neuronal staining was localized to the vestibular nuclei and to other cell bodies mainly in the dorsolateral regions. In the cerebellum, staining was noted mainly in the deepr cerebellar nuclei and in the Purkinje cells. Immunostaining in the cerebral cortex was localized to the cingulate cortex and the primary olfactory cortex. Light staining was present in the endopiriform cortex and in scattered neurons adjacent to the external capsule. In the olfactory bulbs light neuronal staining was mainly associated with the mitral cell layer. The widespread distribution of angiotensinogen-like immunoreactivity supports the view that it is synthesized in the central nervous system and forms part of a brain renin-angiotensin system. In addition, its presence at sites other than those normally associated with the control of blood pressure and fluid and electrolyte homeostasis suggests that its involvement may not be limited to these regulatory functions.