Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development

Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development
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转基因烟草中ipt基因的花药特异性表达及其对植株发育的影响

DOI:
10.1023/a:1015692127101
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发表时间:
2002-06
影响因子:
3
通讯作者:
Li Guofeng
Li Guofeng
中科院分区:
生物学4区
文献类型:
--
作者:
Geng Sa;Ma Mi;Ye Hechun;Li Guofeng

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将来自根癌农杆菌 T-DNA 的异戊烯基转移酶 (ipt) 基因置于在花药中特异性表达的 TA29 启动子的控制下。将嵌合TA29-ipt基因转移到烟草植物中。开花期间,转基因植物花药中ipt基因的mRNA积累,叶、花瓣、雌蕊和雄蕊中iPA+iPs的水平比野生型植物增加3-4倍。这种细胞分裂素的增加影响了发育的各个方面,表明通过使用TA29-ipt基因的花药特异性表达来改变内源细胞分裂素水平影响了转基因植物的形态、花器官系统和繁殖力。
Isopentenyl transferase (ipt) gene from Agrobacterium tumefaciens T-DNA was placed under the control of a TA29 promoter which expresses specifically in anther. The chimeric TA29-ipt gene was transferred to tobacco plants. During flowering, mRNA of the ipt gene in the anthers of the transgenic plants accumulated and the level of iPA + iPs increased 3–4-fold in the leaves, petals, pistils, and stamens compared with those in the wild type plants. This cytokinin increase affected various aspects in development indicating that the alterations of endogenous cytokinin level by using anther-specific expression of the TA29-ipt gene affected morphology, floral organ systems and reproductivity of the transgenic plants.
植物激素突变体
DOI: 10.1007/bf00213038
发表时间: 1993
影响因子: 4.8
作者:
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通讯作者: J. B. Reid
DOI: 10.1007/bf00622145
发表时间: 2004
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DOI: 10.1104/pp.112.1.141
发表时间: 1996-09-01
期刊: PLANT PHYSIOLOGY
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DOI: 10.1007/978-94-009-3585-3_5
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期刊: PLANT CELL
影响因子: 11.6
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