Micromorphologic consequences following perturbation of erythrocyte membranes by trypsin, phospholipase A, lysolecithin, sodium dodecyl sulfate and saponin. A correlated freeze-etching and biochemical study.
Micromorphologic consequences following perturbation of erythrocyte membranes by trypsin, phospholipase A, lysolecithin, sodium dodecyl sulfate and saponin. A correlated freeze-etching and biochemical study.
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胰蛋白酶、磷脂酶 A、溶血卵磷脂、十二烷基硫酸钠和皂苷扰动红细胞膜后的微形态后果。
DOI：
10.1016/0005-2736(72)90037-5
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
H. Knüfermann
中科院分区：
文献类型：
--
作者：
V. Speth;D. Wallach;E. Weidekamm;H. Knüfermann
1.1.用磷脂酶A和低水平的皂苷处理红细胞膜会引起冷冻蚀刻制剂断裂面的交替，使得暴露的膜面被一系列由粘附膜材料组成的斑块覆盖。2.2．增加皂苷、溶血卵磷脂和十二烷基硫酸钠的水平会降低膜切向破裂的数量，可能是因为膜中极性力和非极性力之间平衡的改变使得通常由膜中疏水区域引导的破裂面遵循除沿着膜平面之外的方向。3.3。胰蛋白酶还在其作用位点产生新的极性基团，但不影响断裂面。4.4．我们的数据表明断裂面的交替优先发生在脂质域中。
1.1. Treatment of erythrocyte membranes with phospholipase A and low levels of saponin induces alternation in the fracture plane of freeze-etch preparations in such a way, that exposed membrane faces are covered with an array of plaques made up of adherent membrane material.2.2. Increasing levels of saponin, lysolecithin and sodium dodecyl sulfate lower the number of membranes fracturing tangentially, presumably because the altered balance between polar and apolar forces in the membranes allows the fracture plane, normally guided by hydrophobic regions in the membrane, to follow directions other than along the membrane plane.3.3. Trypsin also generates new polar groups at its site of action, but does not influence the plane of fracture.4.4. Our data suggest that alternations of the fracture plane occur preferentially in lipid domains.