Experimental induction of alopecia areata-like hair loss in C3H/HeJ mice using full-thickness skin grafts
Experimental induction of alopecia areata-like hair loss in C3H/HeJ mice using full-thickness skin grafts
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DOI：
10.1046/j.1523-1747.1998.00380.x
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发表时间：
1998-11-01
影响因子：
6.5
通讯作者：
Sundberg, JP
中科院分区：
文献类型：
--
作者：
McElwee, KJ;Boggess, D;Sundberg, JP
C3 H/HeJ小鼠的斑秃样脱发为人类斑秃病的研究提供了一个很好的模型。通过交换全层皮肤移植物，研究了正常毛发C3 H/HeJ小鼠在早期或连续传代AA表型时诱导小鼠AA的潜力。从正常雄性和雌性C3 H/HeJ或严重联合免疫缺陷C3 H/SmnC Prkdc（scid）/J小鼠的皮肤移植到AA感染的C3 H/HeJ小鼠上变得发炎并掉毛（28/28）。在组织相容性C3 H/OuJ小鼠（4/13）和正常C3 H/HeJ小鼠（4/17在70天）中，AA感染的C3 H/HeJ小鼠的成功移植诱导掉毛。AA表型从诱导的AA小鼠连续传递到正常的C3 H/HeJ小鼠（9/9）。从AA影响的C3 H/HeJ小鼠到C3 H/SmnC Prkdc（scid）/J小鼠的移植物导致脱色的毛纤维再生和滤泡周围中性粒细胞和嗜酸性粒细胞浸润，但没有毛发脱落（15/15）。假移植不诱导AA（10个都没有）。AA可以从AA影响的C3 H/HeJ小鼠连续转移到正常同窝出生的小鼠和C3 H/OuJ小鼠的发现表明，可以用合适的刺激诱导针对毛囊的免疫应答。相反，来自正常C3 H/HeJ、C3 H/SmnC Prkdc（scid）/J小鼠的皮肤移植物由于淋巴细胞而不是嗜中性粒细胞和嗜酸性粒细胞介导的炎症而迅速脱毛。该AA诱导方法可重复地提供大量AA受影响的小鼠以研究人类AA的发病机制和治疗。
Alopecia areata (AA)-like hair loss in C3H/HeJ mice provides an excellent model for human AA disease research. The potential to induce mouse AA in normal haired C3H/HeJ mice at an early age or serially passage the AA phenotype was investigated by exchange of full-thickness skin grafts. Skin grafts from normal male and female C3H/HeJ, or severe combined immunodeficient C3H/SmnC Prkdc(scid)/J, mice onto AA-affected C3H/HeJ mice became inflamed and lost hair (28 of 28), Successful grafts front AA-affected C3H/HeJ mice induced hair loss in histocompatible C3H/OuJ mice (four of 13) and normal C3H/HeJ mice dependent on age (four of 17 at 70 d). The AA phenotype was serially transmitted from induced AA mice to normal C3H/HeJ mice (nine of nine), Grafts from AA-affected C3H/HeJ mice onto C3H/SmnC Prkdc(scid)/J mice resulted in depigmented hair fiber regrowth and perifollicular neutrophil and eosinophil infiltrates but no hair loss (15 of 15). Sham grafting did not induce AA (none of 10), The finding that AA can be serially transferred from AA-affected C3H/HeJ mice to normal littermates and C3H/OuJ mice, indicates that an immune response against hair follicles can be induced with suitable stimuli. Conversely, skin grafts from normal C3H/HeJ, of C3H/SmnC Prkdc(scid)/J, mice rapidly lose hair due to lymphocyte, but not neutrophil and eosinophil, mediated inflammation. This AA induction method reproducibly provides large numbers of AA-affected mice to study the pathogenesis and treatment of human AA.