Injectable and Degradable pH-Responsive Hydrogels via Spontaneous Amino-Yne Click Reaction
Injectable and Degradable pH-Responsive Hydrogels via Spontaneous Amino-Yne Click Reaction
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通过自发氨基-Yne 点击反应开发可注射且可降解的 pH 响应水凝胶，ACS Applied Materials
DOI：
10.1021/acsami.7b18141
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发表时间：
2018-01-10
影响因子：
9.5
通讯作者：
Jiang, Xulin
中科院分区：
文献类型：
--
作者：
Huang, Jiachang;Jiang, Xulin
可注射水凝胶在组织再生和局部药物递送应用中引起了越来越多的关注。目前用于制备可注射水凝胶的点击反应通常需要光引发剂或催化剂，其可能是有毒的并且可能涉及前体的复杂合成。在这里，我们报告了一种简便和廉价的方法来制备可注射和可降解的水凝胶，通过自发的氨基炔点击反应，而不使用任何引发剂或催化剂在生理条件下的基础上遥爪缺电子的聚乙二醇二丙酸酯和水溶性的市售羧甲基壳聚糖（CMC）。通过改变CMC浓度和化学计量比，可以调节水凝胶的凝胶化时间、力学性能和降解速率。结果表明，该水凝胶具有可逆的pH诱导溶胶-凝胶转变，并具有pH控制的药物释放行为，并对其机理进行了探讨。体外细胞毒性试验和体内原位注射研究表明，CMC基水凝胶具有良好的凝胶形成性、无毒性和良好的组织生物相容性。因此，通过自发的氨基炔点击反应制备的可生物降解和可注射的水凝胶在组织工程和其他生物医学领域具有潜在的应用前景。
Injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications. Current click reactions for preparing injectable hydrogels often require a photoinitiator or catalyst, which may be toxic and may involve complex synthesis of precursors. Here, we report a facile and inexpensive method to prepare injectable and degradable hydrogels via spontaneous amino yne click reaction without using any initiator or catalyst under physiological conditions based on telechelic electron-deficient dipropiolate ester of polyethylene glycol and water-soluble commercially available carboxymethyl chitosan (CMC). The gelation time, mechanical property, and degradation rate of the hydrogels could be adjusted by varying CMC concentrations and stoichiometric ratios. The reversible pH-induced sol gel transitions of the hydrogel are presented and the pH-controlled drug release behaviors are demonstrated, of which the mechanism is discussed. In vitro cytotoxicity assays and in vivo in situ injection study of the CMC-based hydrogels showed favorable gel formation, nontoxicity, and good tissue biocompatibility. Therefore, these biodegradable and injectable hydrogels prepared by spontaneous amino-yne click reaction hold potential for tissue engineering and other biomedical