IN-VITRO GUIDANCE OF RETINAL GANGLION-CELL AXONS BY RAGS, A 25 KDA TECTAL PROTEIN RELATED TO LIGANDS FOR EPH RECEPTOR TYROSINE KINASES
IN-VITRO GUIDANCE OF RETINAL GANGLION-CELL AXONS BY RAGS, A 25 KDA TECTAL PROTEIN RELATED TO LIGANDS FOR EPH RECEPTOR TYROSINE KINASES
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DOI：
10.1016/0092-8674(95)90425-5
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发表时间：
1995-08-11
期刊：
影响因子：
64.5
通讯作者：
BONHOEFFER, F
中科院分区：
文献类型：
--
作者：
DRESCHER, U;KREMOSER, C;BONHOEFFER, F
以前的体外实验的结果表明，糖基磷脂酰肌醇（GP1）锚定蛋白可能发挥重要作用，在地形有序的视网膜顶盖投影的形成过程中的颞视网膜轴突的指导。我们已经纯化并克隆了GPI锚定的25 kDa糖蛋白，其是参与该过程的分子的良好候选物。在视网膜神经节细胞支配神经的过程中，该蛋白在发育中的顶盖后部逐渐表达。在两种不同的体外测定系统中，重组蛋白诱导生长锥塌陷和视网膜神经节细胞轴突排斥。这些现象被观察到的颞以及鼻源的轴突，表明额外的活动可能是必要的，赋予在以前的测定中观察到的鼻颞特异性，我们命名为蛋白RAGS（排斥轴突指导信号），RAGS的序列显示出显着的同源性，最近确定的配体受体酪氨酸激酶的Eph亚家族。
The results of previous in vitro experiments indicate that a glycosylphosphatidylinositol (GPl)-anchored protein may play an important role in the guidance of temporal retinal axons during the formation of the topographically ordered retinotectal projection. We have purified and cloned a GPl-anchored, 25 kDa glycoprotein that is a good candidate for a molecule involved in this process. During the time of innervation by retinal ganglion cells, this protein is gradedly expressed in the posterior part of the developing tectum, In two different in vitro assay systems, the recombinant protein induces growth cone collapse and repulsion of retinal ganglion cell axons. These phenomena are observed for axons of temporal as well as nasal origin, indicating that an additional activity may be necessary to confer the nasotemporal specificity observed in previous assays, We named the protein RAGS (for repulsive axon guidance signal), The sequence of RAGS shows significant homology to recently identified ligands for receptor tyrosine kinases of the Eph subfamily.