Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα
Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα
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DOI：
10.3892/mmr.2012.1070
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Zuo, Ji
中科院分区：
文献类型：
--
作者：
Guo, Weiwei;Yang, Ling;Zuo, Ji
葡萄糖调节蛋白75（Grp 75），也称为mortalin/mthsp 70/PBP 74，是热休克蛋白70（HSP 70）家族的成员。Grp 75可以保护细胞免受应激诱导的损伤。先前的研究表明，低剂量电离辐射（IR）可上调Grp 75的表达。为了评估Grp 75对细胞增殖响应IR的保护作用，Grp 75在大鼠肾上腺嗜铬细胞瘤细胞系（PC 12）中过表达。结果表明，Grp 75过表达使PC 12细胞对IR介导的细胞损伤脱敏。此外，拓扑异构酶II α（Topo II α）的表达下调后，γ射线IR的PC 12细胞。Grp 75过表达对Topo II α表达的影响进行了检查。结果表明，Grp 75过表达逆转了IR对Topo ha表达的抑制作用。总之，数据表明Grp 75过表达通过维持Topo II α的表达来减弱PC 12细胞中IR诱导的损伤。
Glucose-regulated protein 75 (Grp75), also known as mortalin/mthsp70/PBP74, is a member of the heat-shock protein 70 (HSP70) family. Grp75 is known to protect cells from stress-induced injury. Previous studies have shown that the expression of Grp75 is upregulated by a low dose of ionizing radiation (IR). To evaluate the protective role of Grp75 on cell proliferation in response to IR, Grp75 was over-expressed in a rat adrenal pheochromocytoma cell line (PC12). It was revealed that Grp75 overexpression desensitized PC12 cells to IR-mediated cell injury. In addition, the expression of topoisomerase II alpha (Topo II alpha) was downregulated in PC12 cells following gamma-ray IR. The effect of Grp75 overexpression on Topo II alpha expression was examined. It was revealed that Grp75 overexpression reversed the inhibitory effect of IR on Topo ha expression. In conclusion, the data indicated that Grp75 overexpression attenuates IR-induced injury in PC12 cells by maintaining the expression of Topo II alpha.