Apical cell escape from the neuroepithelium and cell transformation during terminal lip fusion in the house shrew embryo
Apical cell escape from the neuroepithelium and cell transformation during terminal lip fusion in the house shrew embryo
复制标题
家鼩胚胎终唇融合过程中顶端细胞从神经上皮逃逸和细胞转化
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
K. Eto
中科院分区：
文献类型：
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作者：
K. Yasui;Y. Ninomiya;N. Osumi‐Yamashita;S. Shibanai;K. Eto
家鼠胚胎的脑室腔内和头神经管融合末端唇的管腔表面有许多细胞。在全胚胎培养系统中，用光学显微镜、电子显微镜和DiI标记的方法研究了这些细胞的起源和命运。细胞出现在11A期，并持续到12A期。上皮细胞逃逸和DiI标记分析表明，大多数细胞似乎起源于神经上皮。管腔表面的细胞有时出现凋亡特征，但不发生吞噬作用。一些逃逸的细胞似乎迁移到前脑神经孔的腹侧部分，并将自己插入其中。其他的则从管腔表面分离并漂浮到管腔内。看起来漂浮的细胞要么自溶，要么变成巨噬细胞样细胞，后者的选择得到DiI标记结果的支持。巨噬细胞样细胞主动吞噬其他变性细胞和凋亡小体。这些观察结果表明，细胞的顶端逃逸可能在末端唇部融合过程中神经褶的重塑中起重要作用，早期的神经上皮细胞可能有可能成为具有强烈吞噬活性的细胞，如巨噬细胞。
The house shrew embryo has many cells in the ventricular lumen and on the luminal surface of the fusing terminal lip of the cephalic neural tube. The origin and fate of these cells were studied by means of light and electron microscopy, and by DiI labeling in a whole-embryo culture system. The cells appeared at stage 11A and persisted until stage 12A. Most of the cells seemed to originate from the neuroepithelium, as shown by frequent observations of epithelial cell escape and DiI labeling analysis. The cells on the luminal surface sometimes showed apoptotic features, but were not subjected to phagocytosis. Some of the escaping cells seemed to migrate to the ventral part of the prosencephalic neuropore and insert themselves into it. Others separated from the luminal surface and floated into the lumen. It seems likely that the floating cells either become autolyzed, or else change into macrophage-like cells, the latter alternative being supported by the results of DiI labeling. The macrophage-like cells actively phagocytosed the other degenerating cells and apoptotic bodies. These observations suggest that the apical escape of cells may play an important role in the remodeling of the neural fold during the terminal lip fusion, and that early neuroepitheial cells may have the potential to become cells with vigorous phagocytic activity, like macrophages.