Proteomics analysis reveals marker proteins for minor vein initiation in rice leaf
Proteomics analysis reveals marker proteins for minor vein initiation in rice leaf
复制标题
蛋白质组学分析揭示了水稻叶片小静脉起始的标记蛋白
DOI：
10.1007/s10142-018-0612-1
复制
发表时间：
2018-09-01
影响因子：
2.9
通讯作者：
Han, Xiao
中科院分区：
文献类型：
--
作者：
Feng, Dan;Wang, Yanwei;Han, Xiao
叶脉在资源供给和光合转运中起着关键作用；因此，它被认为是作物育种的重要农业性状。水稻的小脉从基部到叶尖沿叶片各部分平行生长。为了解水稻叶片小叶脉的发育过程，通过解剖分析揭示了水稻叶片小叶脉的形成和发育过程。小脉形成的频率从叶基到叶尖呈下降趋势。利用itraq对水稻叶片进行了蛋白质组学分析。与光合作用和碳固定相关的蛋白质在叶片中部积累水平较高。此外，参与蔗糖降解和淀粉合成的标记蛋白分别积累在小叶脉的起始和成熟部位。这表明在小叶脉的起始和成熟部分，源库活性在光合物质转运方面是不同的。所鉴定的蛋白是水稻叶片小叶脉形成的候选标记物。
Leaf veins play a critical role in resource supplication and photosynthate translocation; thus, it is considered as an important agricultural trait for crop breeding. The rice minor veins are parallelly grown along all the parts of the leaf from base to tip. To understand the process of minor vein development, anatomy analysis was performed to reveal the initiation and development of minor veins in rice leaf. The frequency of minor vein initiation follows a decreased tendency from leaf base to tip. An iTRAQ-based proteomics analysis was performed in rice leaf sections. Photosynthesis- and carbon fixation-related proteins accumulated a high level in the middle part of leaves. Furthermore, marker proteins involved in sucrose degradation and starch synthesis were accumulated into initiation and mature parts of minor veins, respectively. It suggests a different source-sink activity in the initiation and mature parts of minor veins in terms of photosynthate translocation. The identified proteins are candidate markers for small vein initiation in rice leaves.